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MAB5606Z Anti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free)

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MAB5606Z
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, F, BIH(P), ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5606Z
      ReplacesMAB5606
      DescriptionAnti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free)
      Alternate Names
      • GD1a Ganglioside
      Background InformationGangliosides are sialic acid-containing oligoglycosylceramides. The German scientist Ernst Klenk used the term ganglioside in 1942 to refer to lipids isolated from ganglions. Gangliosides are grouped based on the number of sialic acid residues per molecule, GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, GQ contain four sialic acid residues. Glycolipids constitute >80% of mammalian brain glycans, with gangliosides being the major glycans in nerve cells. Among the hundreds of ganglioside structures, GM1a, GD1a, GD1b and GT1b comprise up to 97% of gangliosides in the human brain. These four gangliosides share the same neutral tetrasaccharide core (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1′Cer), but differ from each other by their sialic acid residue(s). GM1a and GD1b contain one or two sialic acids on the internal galactose, respectively, and no sialic acid on the terminal galactose. The St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III sialyltransferase, respectively) are largely responsible for ganglioside terminal α2-3 sialylation of GM1a and GD1b to produce GD1a and GT1b in the brain. Brain GD1a and GT1b in St3gal2-null mice is about half the level in wild-type mice, while GD1a and GT1b level in St3gal2/3-double-null mice is less than 5% of the level in wild-type mice. St3gal2/3-double-null mice are half the weight of wild-type mice at weaning and display early hindlimb dysreflexia.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing PBS with no azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free) is an antibody against GD1a Ganglioside for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA).
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Enzyme Immunoassay (ELISA)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GD1a Ganglioside in human prostate tissue.
      ELISA Analysis: Target specificity of a representative lot was determined by ELISA analysis (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
      Immunohistochemistry Analysis: A representative lot detected GD1a immunoreactivity in fresh-frozenmid-sagittal brain sections from wild-type and St3gal2/3-double-null mice (Sturgill, E.R., et al. (2012). Glycobiology.22(10):1289-1301).
      Immunohistochemistry Analysis: A representative lot detected GD1a immunoreactivity in various fish brain regions using free-floating sections (Viljetić, B., et al. (2012). Biochim Biophys Acta. 1820(9):1437-1443).
      Immunohistochemistry Analysis: A representative lot detected GD1a in fresh-frozen human and rat brain sections by both fluorescent and non-fluorescent immunohistochemistry (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
      Immuno-overlay Assay: A representative lot detected bovine brain GD1a, as well as GD1a in human ventral and dorsal root gangliosides extracts (Gong, Y., et al. (2002). Brain. 125(Pt 11):2491-2506).
      Biological Information
      ImmunogenKLH-conjugated GD1a ganglioside.
      CloneGD1a-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityGD1a ganglioside. The antibody shows approximately 10% reactivity to GalNAc-GD1a and GT1aα. Preferentially binds to motor myelinated fibers in cross-sections of ventral roots and sciatic nerves.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Fish
      • Bovine
      Species Reactivity NoteHuman, Mouse, Rat, Fish, Bovine. Predicted to react with Vertebrates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human heart tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GD1a Ganglioside in human heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB5606Z 04055977294736

      Documentation

      Anti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GD1a Ganglioside Antibody, clone GD1a-1 (Azide Free) Certificates of Analysis

      TitleLot Number
      Anti-GD1a Ganglioside, -Q2613633 Q2613633
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) - 3316668 3316668
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) - 3733773 3733773
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) - 3808633 3808633
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) - 3922545 3922545
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) - 4069727 4069727
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) -2829971 2829971
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) Monoclonal Antibody 3137649
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) Monoclonal Antibody 2906238
      Anti-GD1a Ganglioside, clone GD1a-1 (Azide Free) Monoclonal Antibody 3091903

      References

      Reference overviewApplicationSpeciesPub Med ID
      Biosynthesis of the major brain gangliosides GD1a and GT1b.
      Sturgill, ER; Aoki, K; Lopez, PH; Colacurcio, D; Vajn, K; Lorenzini, I; Majić, S; Yang, WH; Heffer, M; Tiemeyer, M; Marth, JD; Schnaar, RL
      Glycobiology  22  1289-301  2012

      Show Abstract
      22735313 22735313
      Distribution of mono-, di- and trisialo gangliosides in the brain of Actinopterygian fishes.
      Viljetić, B; Labak, I; Majić, S; Stambuk, A; Heffer, M
      Biochimica et biophysica acta  1820  1437-43  2012

      Show Abstract
      22206893 22206893
      Localization of major gangliosides in the PNS: implications for immune neuropathies.
      Gong, Y; Tagawa, Y; Lunn, MP; Laroy, W; Heffer-Lauc, M; Li, CY; Griffin, JW; Schnaar, RL; Sheikh, KA
      Brain : a journal of neurology  125  2491-506  2002

      Show Abstract
      Immunohistochemistry (Tissue)Rat, Human, Mouse12390975 12390975

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