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MABS819 Anti-GAPDH (CT) Antibody, clone RM114

MABS819
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS819
      DescriptionAnti-GAPDH (CT) Antibody, clone RM114
      Alternate Names
      • Glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      • GAPDH
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationThis Anti-GAPDH (CT) Antibody, clone RM114 is validated for use in Western Blotting, Immunocytochemistry for the detection of GAPDH (CT).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected GAPDH (CT) in A431 cells.

      Biological Information
      ImmunogenA peptide corresponding to the C-terminus of GAPDH.
      EpitopeC-terminus
      CloneRM114
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GAPDH
      • GAPD
      • CDABP0047
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~39 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GAPDH (CT) in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-GAPDH (CT) Antibody, clone RM114 Certificates of Analysis

      TitleLot Number
      Anti-GAPDH (CT), clone RM114 -VP1811300 VP1811300
      Anti-GAPDH (CT), clone RM114 - VP1912273 VP1912273
      Anti-GAPDH (CT), clone RM114 -QVP1503207 QVP1503207
      Anti-GAPDH (CT), clone RM114 Monoclonal Antibody VP1807279
      Anti-GAPDH (CT), clone RM114 Monoclonal Antibody VP1706195

      Brochure

      Title
      New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody (EMD)

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage. (EMD)

      Posters

      Title
      Post-Translational Modifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies