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ABS16 Anti-GAPDH Antibody

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ABS16
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS16
      DescriptionAnti-GAPDH Antibody
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase, GAPDH
      • G3PDH
      • GAPD
      • OK/SW-cl.12
      • MGC88685
      • G3PD
      • EC 1.2.1.12
      Background InformationGlyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is considered as a 'house-keeping' enzyme that catalyzes the synthesis of 1,3-biphosphoglycerate. It is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Hence, it plays a role in both glycolysis and nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1, which contains a nuclear localization signal. In the nucleus it participates in transcription, RNA transport, DNA replication and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAPDH in NIH3T3 and L6 cell lysates.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 90 amino acids from the C-terminal region of human Glyceraldehyde-3-phosphate dehydrogenase.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). It targets an epitope within the C-terminal region.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Guinea Pig based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

      SIZE: 335 amino acids; 36053 Da

      SUBUNIT: Homotetramer. Binds PRKCI.

      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

      SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight~36 kDa observed; 36.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:4,000 dilution of this antibody detected GAPDH in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-GAPDH Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GAPDH Antibody Certificates of Analysis

      TitleLot Number
      Anti-GAPDH 3076454
      Anti-GAPDH - 2128266 2128266
      Anti-GAPDH - 2384012 2384012
      Anti-GAPDH - 2459656 2459656
      Anti-GAPDH - 2059942 2059942
      Anti-GAPDH - 2201477 2201477
      Anti-GAPDH - 2272157 2272157
      Anti-GAPDH - 2491262 2491262
      Anti-GAPDH - 2535325 2535325
      Anti-GAPDH - 3129236 3129236

      References

      Reference overviewApplicationSpeciesPub Med ID
      Activation of peroxisome proliferator-activated receptor γ ameliorates monocrotaline-induced pulmonary arterial hypertension in rats.
      Xie, X; Wang, G; Zhang, D; Zhang, Y; Zhu, Y; Li, F; Li, S; Li, M
      Biomedical reports  3  537-542  2015

      Show Abstract
      26171162 26171162
      AduPARE1A and gemcitabine combined treatment trigger synergistic antitumor effects in pancreatic cancer through NF-κB mediated uPAR activation.
      Maliandi, MV; Mato-Berciano, A; Sobrevals, L; Roué, G; José, A; Fillat, C
      Molecular cancer  14  146  2015

      Show Abstract
      26227809 26227809
      Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
      Tsai, HL; Deng, WP; Lai, WF; Chiu, WT; Yang, CB; Tsai, YH; Hwang, SM; Renshaw, PF
      PloS one  9  e104937  2014

      Show Abstract
      ImmunofluorescenceHuman25170755 25170755
      Comparison of the anti-prion mechanism of four different anti-prion compounds, anti-PrP monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine, and U18666A, in prion-infected mouse neuroblastoma cells.
      Yamasaki, T; Suzuki, A; Hasebe, R; Horiuchi, M
      PloS one  9  e106516  2014

      Show Abstract
      25181483 25181483
      Receptor-interacting protein kinases modulate noise-induced sensory hair cell death.
      Zheng, HW; Chen, J; Sha, SH
      Cell death & disease  5  e1262  2014

      Show Abstract
      Western Blotting24874734 24874734
      Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.
      Miyata, K; Yotsumoto, F; Nam, SO; Odawara, T; Manabe, S; Ishikawa, T; Itamochi, H; Kigawa, J; Takada, S; Asahara, H; Kuroki, M; Miyamoto, S
      Cancer medicine  3  1159-69  2014

      Show Abstract
      Western Blotting25060396 25060396
      Mitochondrial peroxiredoxin 3 regulates sensory cell survival in the cochlea.
      Chen, FQ; Zheng, HW; Schacht, J; Sha, SH
      PloS one  8  e61999  2013

      Show Abstract
      23626763 23626763
      The miR-183/Taok1 target pair is implicated in cochlear responses to acoustic trauma.
      Patel, M; Cai, Q; Ding, D; Salvi, R; Hu, Z; Hu, BH
      PloS one  8  e58471  2013

      Show Abstract
      23472202 23472202
      Traumatic noise activates Rho-family GTPases through transient cellular energy depletion.
      Chen, FQ; Zheng, HW; Hill, K; Sha, SH
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  12421-30  2012

      Show Abstract
      22956833 22956833
      Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.
      Greciano, PG; Moyano, JV; Buschmann, MM; Tang, J; Lu, Y; Rudnicki, J; Manninen, A; Matlin, KS
      Molecular biology of the cell  23  121-36  2012

      Show Abstract
      22031290 22031290

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies