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AB2302 Anti-GAPDH Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Eq, CaWBChAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB2302
      DescriptionAnti-GAPDH Antibody
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      Product Information
      FormatAffinity Purified
      • HEK293 cell lysate
      PresentationPurified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.
      EpitopeWARS interacting region
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes GAPDH.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Canine
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with equine and canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

      SIZE: 335 amino acids; 36053 Da

      SUBUNIT: Homotetramer. Binds PRKCI.

      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

      SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight~ 38 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 and PC12 cell lysate.

      Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-GAPDH Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-GAPDH Antibody Certificates of Analysis

      TitleLot Number
      Anti-GAPDH - 2424824 2424824
      Anti-GAPDH - 1944115 1944115
      Anti-GAPDH - 1986173 1986173
      Anti-GAPDH - 2029184 2029184
      Anti-GAPDH - 2105272 2105272
      Anti-GAPDH - 2181288 2181288
      Anti-GAPDH - 2276425 2276425
      Anti-GAPDH - 2327505 2327505
      Anti-GAPDH - 3022890 3022890
      Anti-GAPDH - 3170836 3170836


      Reference overviewApplicationSpeciesPub Med ID
      Nkx2.2 and Nkx2.9 are the key regulators to determine cell fate of branchial and visceral motor neurons in caudal hindbrain.
      Jarrar, W; Dias, JM; Ericson, J; Arnold, HH; Holz, A
      PloS one  10  e0124408  2015

      Show Abstract
      25919494 25919494
      hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.
      Thompson, PJ; Dulberg, V; Moon, KM; Foster, LJ; Chen, C; Karimi, MM; Lorincz, MC
      PLoS genetics  11  e1004933  2015

      Show Abstract
      Western Blotting25611934 25611934
      Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling.
      Kruiswijk, F; Hasenfuss, SC; Sivapatham, R; Baar, MP; Putavet, D; Naipal, KA; van den Broek, NJ; Kruit, W; van der Spek, PJ; van Gent, DC; Brenkman, AB; Campisi, J; Burgering, BM; Hoeijmakers, JH; de Keizer, PL
      Oncogene  2015

      Show Abstract
      26279295 26279295
      Follistatin promotes adipocyte differentiation, browning, and energy metabolism.
      Braga, M; Reddy, ST; Vergnes, L; Pervin, S; Grijalva, V; Stout, D; David, J; Li, X; Tomasian, V; Reid, CB; Norris, KC; Devaskar, SU; Reue, K; Singh, R
      Journal of lipid research  55  375-84  2014

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      Western BlottingMouse24443561 24443561
      Acid-sensing ion channels contribute to synaptic transmission and inhibit cocaine-evoked plasticity.
      Kreple, CJ; Lu, Y; Taugher, RJ; Schwager-Gutman, AL; Du, J; Stump, M; Wang, Y; Ghobbeh, A; Fan, R; Cosme, CV; Sowers, LP; Welsh, MJ; Radley, JJ; LaLumiere, RT; Wemmie, JA
      Nature neuroscience  17  1083-91  2014

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      24952644 24952644
      CNS expression of murine fragile X protein (FMRP) as a function of CGG-repeat size.
      Ludwig, AL; Espinal, GM; Pretto, DI; Jamal, AL; Arque, G; Tassone, F; Berman, RF; Hagerman, PJ
      Human molecular genetics  23  3228-38  2014

      Show Abstract
      24463622 24463622
      Genetic suppression of transgenic APP rescues Hypersynchronous network activity in a mouse model of Alzeimer's disease.
      Born, HA; Kim, JY; Savjani, RR; Das, P; Dabaghian, YA; Guo, Q; Yoo, JW; Schuler, DR; Cirrito, JR; Zheng, H; Golde, TE; Noebels, JL; Jankowsky, JL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  3826-40  2014

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      24623762 24623762
      Piericidin A aggravates Tau pathology in P301S transgenic mice.
      Höllerhage, M; Deck, R; De Andrade, A; Respondek, G; Xu, H; Rösler, TW; Salama, M; Carlsson, T; Yamada, ES; Gad El Hak, SA; Goedert, M; Oertel, WH; Höglinger, GU
      PloS one  9  e113557  2014

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      25437199 25437199
      DNA methylation impairs TLR9 induced Foxp3 expression by attenuating IRF-7 binding activity in fulminant type 1 diabetes.
      Wang, Zhen, et al.
      J. Autoimmun., 41: 50-9 (2013)  2013

      Show Abstract
      23490285 23490285
      Profiling murine tau with 0N, 1N and 2N isoform-specific antibodies in brain and peripheral organs reveals distinct subcellular localization, with the 1N isoform being enriched in the nucleus.
      Liu, C; Götz, J
      PloS one  8  e84849  2013

      Show Abstract
      24386422 24386422

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      Life Science Research > Antibodies and Assays > Primary Antibodies