341286 Anti-Farnesyl Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of Species Rb Polyclonal Antibody

Pricing & Availability

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341286-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes farnesyl. Also recognizes KLH.
      Catalogue Number341286
      Brand Family Calbiochem®
      Application Data
      Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
      References
      ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
      Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
      Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
      Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
      Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunofluorescence
      Application NotesELISA (1:1000)
      Immunofluorescence (1:50)
      Immunoblotting (not recommended; see comments)
      Application CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenfarnesyl cysteine conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Farnesyl Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Farnesyl Rabbit pAb Certificates of Analysis

      TitleLot Number
      341286

      References

      Reference overview
      Baron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
      Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
      Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
      Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
      Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-October-2007 RFH
      ApplicationELISA (1:1000)
      Immunofluorescence (1:50)
      Immunoblotting (not recommended; see comments)
      Application Data
      Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes farnesyl.
      BackgroundPrenylation of proteins is a post-translational modification that is necessary for membrane association of proteins as well as protein-protein interactions. The nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors. Approximately 0.5% of cellular proteins are affected by protein isoprenylation. Farnesyltransferase and geranylgeranyltransferase are the two enzymes involved in catalyzing the attachment of two prenyl groups to the sulfhydryl group of the C-terminal cysteine groups. These enzymes transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C= Cysteine, A1 & A2 = aliphatic amino acids). Some of the proteins that contain the C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins, and the γ-subunit of trimeric G proteins.
      HostRabbit
      Immunogenfarnesyl cysteine conjugated to KLH
      IsotypeIgG
      Speciesa broad range of species
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
      Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
      Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
      Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
      Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.