ST1579 Anti-FADS1 Mouse mAb (2D9)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1579-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~50-55 kDa FADS1 protein in HeLa cells and human skeletal muscle tissue.
      Catalogue NumberST1579
      Brand Family Calbiochem®
      SynonymsAnti-Fatty Acid Desaturase 1
      Application Data
      Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, Human skeletal muscle tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
      Clone2D9
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-FADS1 Mouse mAb (2D9) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-Fatty Acid Desaturase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-55 kDa FADS1 protein.
      BackgroundFADS1 is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
      Clone2D9
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells, Human skeletal muscle tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review