AB5026 Anti-Enkephalin Antibody, Met

AB5026
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, IHC Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5026
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Enkephalin Antibody, Met
      References
      Product Information
      FormatPurified
      PresentationProtein A/G purified immunoglobulin. Liquid containing 0.1% sodium azide.
      Applications
      ApplicationAnti-Enkephalin Antibody, Met is an antibody against Enkephalin for use in IC, IH.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 2-10μg/mL. See suggested portocols. Optimal working dilutions must be determined by the end user.

      IMMUNOHISTOCHEMISTRY

      Anesthetize animals and perfuse them transcardially as follows*:

      a) Flush with cold (4°C) oxygenated Calcium free Tyrodes

      b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9

      c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)

      * For better visualization of cell bodies immunoreactive for Met5-enkephalin, pretreatment with colchicine 24-48 hours before perfusion may be required (see reference 2)



      Sections

      Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.



      Immunofluorescence

      Dilute Met-enkephalin antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.01% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate for one hour at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.



      Indirect Avidin-Biotin Peroxidase

      Incubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute Met-enkephalin antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.01% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections three times for 15 minutes in PBS and incubate them with 0.03% of diaminobenzidine (DAB) in PBS/0.015% H2O2 for 3-15 minutes at room temperature. Dehydrate sections in grading alcohols (50%, 75%, 80%, 96% and 100%), clean them in xylene and coverslip with DPX or Permount. Sections can be examined using conventional microscopy.





      Important Note: During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.
      Biological Information
      ImmunogenSynthetic Met5-enkephalin peptide (H-Tyr-Gly-Gly-Phe-Met-OH) conjugated through the N-terminal tyrosine to BSA.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityMet5-enkephalin. Cross reactivity: Leu5-enkephalin 5.8%, beta-endorphin <0.01% and beta-Lipotropin <0.01%.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PENK
      • proenkephalin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01210 # Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
      SIZE: 267 amino acids; 30787 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
      SIMILARITY: SwissProt: P01210 ## Belongs to the opioid neuropeptide precursor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Enkephalin Antibody, Met SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Enkephalin Antibody, Met Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-MET-ENKEPHALIN2449196
      RABBIT ANTI-MET-ENKEPHALIN POLYCLONAL ANTIBODY - 23956682395668
      RABBIT ANTI-MET-ENKEPHALIN POLYCLONAL ANTIBODY - 20166142016614
      RABBIT ANTI-MET-ENKEPHALIN POLYCLONAL ANTIBODY - 20808272080827
      RABBIT ANTI-MET-ENKEPHALIN POLYCLONAL ANTIBODY - 22014122201412
      RABBIT ANTI-MET-ENKEPHALIN POLYCLONAL ANTIBODY - 22956372295637

      References

      Reference overviewPub Med ID
      Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.
      Goto, S; Morigaki, R; Okita, S; Nagahiro, S; Kaji, R
      Frontiers in neuroanatomy  9  22  2015

      Show Abstract
      25784860 25784860
      Expression of mu opioid receptor in dorsal diencephalic conduction system: new insights for the medial habenula.
      Gardon, O; Faget, L; Chu Sin Chung, P; Matifas, A; Massotte, D; Kieffer, BL
      Neuroscience  277  595-609  2014

      Show Abstract
      25086313 25086313
      GABAergic inputs from direct and indirect striatal projection neurons onto cholinergic interneurons in the primate putamen.
      Gonzales, KK; Pare, JF; Wichmann, T; Smith, Y
      The Journal of comparative neurology  521  2502-22  2013

      Show Abstract
      23296794 23296794
      An anxiolytic role for CRF receptor type 1 in the globus pallidus.
      Sztainberg, Y; Kuperman, Y; Justice, N; Chen, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  17416-24  2011

      Show Abstract
      22131403 22131403
      Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep.
      Funato, H; Sato, M; Sinton, CM; Gautron, L; Williams, SC; Skach, A; Elmquist, JK; Skoultchi, AI; Yanagisawa, M
      Proceedings of the National Academy of Sciences of the United States of America  107  18155-60  2010

      Show Abstract Full Text Article
      20921407 20921407
      Differential regulation of morphine antinociceptive effects by endogenous enkephalinergic system in the forebrain of mice.
      Chen, TC; Cheng, YY; Sun, WZ; Shyu, BC
      Molecular pain  4  41  2008

      Show Abstract
      18826595 18826595
      Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres.
      Fie Juhl,Jens Hannibal,Jan Fahrenkrug
      Cell and tissue research  329  2007

      Show Abstract
      17503087 17503087
      Expression of protein kinase C-substrate mRNAs in the basal ganglia of adult and infant macaque monkeys.
      Noriyuki Higo, Takao Oishi, Akiko Yamashita, Yumi Murata, Keiji Matsuda, Motoharu Hayashi
      The Journal of comparative neurology  499  662-76  2006

      Show Abstract
      17029258 17029258
      Huntingtin distribution among striatal output neurons of normal rat brain
      Fusco, F.R. et al.
      Neuroscience Letters , 339:53-56 (2003)  2003

      12618299 12618299
      Neuropeptides.
      Moore, M R and Black, P M
      Neurosurgical review, 14: 97-110 (1991)  1991

      Show Abstract
      1870724 1870724

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      Categories

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