MAB5276 Anti-Endorphin β Antibody, clone 3-E7

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MAB5276
40 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Po ELISA, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5276
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Endorphin β Antibody, clone 3-E7
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Endorphin Antibody, β, clone 3-E7 is validated for use in ELISA, IH for the detection of Endorphin.
      Key Applications
      • ELISA
      • Immunohistochemistry
      Application NotesELISA Immunohistochemistry

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic human beta-endorphin coupled with poly-lysine or thyroglobulin (Gramsch et al., 1983; Meo et al., 1983) (H-Tyr-Gly-Gly-Phe sequence).
      Clone3-E7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with the amino terminal H-Tyr-Gly-Gly-Phe sequence of human O-endorphin. Therefore, a high cross-reactivity with homologues of identical sequence, such as [Met]-enkephalin and [Leu]-enkephalin and a number of opioid peptides, occurs; the antibody also reacts with swine and camel O-endorphin. However, the antibody does not recognize human beta-lipotropin and N-acetyl-beta-endorphin (Gramsch et al., 1983; Meo et al., 1983; Herz et al., 1982).
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
      Gene Symbol
      • POMC
      • pro-ACTH-endorphin
      • adrenocorticotropin
      • beta-LPH
      • POC
      • gamma-LPH
      • LPH
      • beta-endorphin
      • corticotropin
      • ACTH
      • alpha-MSH
      • beta-MSH
      • pro-opiomelanocortin
      • proopiomelanocortin
      • Pro-opiomelanocortin
      • NPP
      • corticotropin-lipotrophin
      • MSH
      • met-enkephalin
      • CLIP
      • gamma-MSH
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.
      SIZE: 267 amino acids; 29424 Da
      TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.
      DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.
      SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size40 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Endorphin β Antibody, clone 3-E7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Endorphin β Antibody, clone 3-E7 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-ENDORPHIN, #946; MONOCLONAL ANTIBODY - 21658332165833
      MOUSE ANTI-ENDORPHIN, B MONOCLONAL ANTIBODY2942537
      MOUSE ANTI-ENDORPHIN, b MONOCLONAL ANTIBODY - 20513432051343
      MOUSE ANTI-ENDORPHIN, b MONOCLONAL ANTIBODY - 21995962199596
      MOUSE ANTI-ENDORPHIN, b MONOCLONAL ANTIBODY - 22059192205919
      MOUSE ANTI-ENDORPHIN, b MONOCLONAL ANTIBODY - 22763462276346
      MOUSE ANTI-ENDORPHIN, b MONOCLONAL ANTIBODY - 23605762360576
      MOUSE ANTI-ENDORPHIN, ß -28761842876184
      MOUSE ANTI-ENDORPHIN, β - 25179292517929
      MOUSE ANTI-ENDORPHIN, β - 28285652828565

      References

      Reference overviewPub Med ID
      Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides.
      Gramsch, C, et al.
      J. Neurochem., 40: 1220-6 (1983)  1983

      Show Abstract
      6187900 6187900
      Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptors.
      Meo, T, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 4084-8 (1983)  1983

      Show Abstract
      6191329 6191329
      Opioid peptide-like immunoreactivity localized in GABAErgic neurons of rat neostriatum and central amygdaloid nucleus.
      Oertel, W H, et al.
      Life Sci., 33 Suppl 1: 73-6 (1983)  1983

      Show Abstract
      6664248 6664248
      Characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides.
      Herz, A, et al.
      Life Sci., 31: 1721-4 (1982)  1982

      Show Abstract
      6296574 6296574

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies