AB5028 Anti-Endorphin β Antibody

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AB5028
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, RIA Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5028
      ReplacesCBL98
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Endorphin β Antibody
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin Liquid. Contains 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Endorphin Antibody, β is an antibody against Endorphin for use in IH, RIA.
      Key Applications
      • Immunohistochemistry
      • Radioimmunoassay
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:500-1:1,000 using immunofluorescence and 1:5,000-1:10,000 for indirect avidin-biotin.



      Not recommended for traditional formalin fixed paraffin tissues. 4% formalin fixed, resin embedded tissues can be used. Antibodies should be diluted in simple solutions (PBS plus 1% BSA) only. Blocking should not contain serum.



      RIA: 1:10,000; sensitivity 120 pg/sample; IC50 - 1 ng/sample)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide (H-Thy-Gly-Gly-Phe-Met-Thr-Ser-Lys-
      Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala-
      Ile-Ile-Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Glu-OH) from human beta-endorphin conjugated to thyroglobulin.
      HostRabbit
      SpecificityBeta-endorphin. Cross reactivity: Met-enkephalin 0.03%, Leu-enkephalin 0.02% and beta-Lipotropin 0.34%.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
      Gene Symbol
      • POMC
      • pro-ACTH-endorphin
      • adrenocorticotropin
      • beta-LPH
      • POC
      • gamma-LPH
      • LPH
      • ACTH
      • corticotropin
      • beta-endorphin
      • beta-MSH
      • alpha-MSH
      • pro-opiomelanocortin
      • proopiomelanocortin
      • Pro-opiomelanocortin
      • corticotropin-lipotrophin
      • NPP
      • MSH
      • met-enkephalin
      • CLIP
      • gamma-MSH
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.
      SIZE: 267 amino acids; 29424 Da
      TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.
      DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.
      SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Endorphin β Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Endorphin β Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY - 21596342159634
      RABBIT ANTI-BETA-ENDORPHIN - 30160193016019
      RABBIT ANTI-BETA-ENDORPHIN -25763292576329
      RABBIT ANTI-BETA-ENDORPHIN -26591762659176
      RABBIT ANTI-BETA-ENDORPHIN -27078182707818
      RABBIT ANTI-BETA-ENDORPHIN -27750632775063
      RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY2913872
      RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY - 19908971990897
      RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY - 20802732080273
      RABBIT ANTI-BETA-ENDORPHIN POLYCLONAL ANTIBODY - 23447152344715

      References

      Reference overviewPub Med ID
      Changes in melanocortin expression and inflammatory pathways in fetal offspring of nonhuman primates fed a high-fat diet.
      B E Grayson,P R Levasseur,S M Williams,M S Smith,D L Marks,K L Grove
      Endocrinology  151  2010

      Show Abstract Full Text Article
      20176722 20176722
      Immunohistochemical detection of progestin receptors in hypothalamic beta-endorphin and substance P neurons of steroid-treated monkeys
      Bethea, C.L. and Widmann, A. A.
      Neuroendocrinology, 63(2):132-141 (1996)  1996

      9053777 9053777
      Immunoelectron microscopy of beta-endorphinergic synaptic innervation of nitric oxide synthase immunoreactive neurons in the dorsal raphe nucleus
      Wang, O.P. and Nakai, Y.
      Brain Research, 684:185-193 (1995)  1995

      7583221 7583221
      Immunocytochemical localization of pro-opiomelanocortin neurons in human brain areas subserving stimulation analgesia.
      Pilcher, W.H. et al.
      J. Neurosurgery, 68(4):621-629 (1988)  1988

      2832554 2832554
      Fixation, fine structure, and immunostaining for neuropeptides: perfusion versus immersion of the neuroendocrine hypothalamus
      Lamberts, R. and Goldsmith, P.C.
      J. Histochem. Cytochem., 34:389-398 (1986)  1986

      2419392 2419392
      Proopiomelanocortin peptide immunocytochemistry in rhesus monkey brain.
      Khachaturian, H, et al.
      Brain Res. Bull., 13: 785-800 (1984)  1984

      Show Abstract
      6099745 6099745
      Immunocytochemistry of endorphins and enkephalins
      Bloom, F.E. and Battenberg, E.L.
      Methods in Enzymology, 103:670-687 (1983)  1983

      6321899 6321899
      Immunocytochemical localization of beta-endorphin (lipotropin C-fragment) in the developing rat spinal cord and hypothalamus
      Haynes, L.W. et al.
      Brain Research, 232(1):115-128 (1982)  1982

      6275955 6275955

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies