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DR1093 Anti-ETV6 Mouse mAb (3B10)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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DR1093-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~55-60 kDa ETV6 protein in Jurkat cells and human placenta tissue.
      Catalogue NumberDR1093
      Brand Family Calbiochem®
      SynonymsAnti-ETS Variant 6
      Application Data
      Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlJurkat cells, Human placenta tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
      Clone3B10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-ETV6 Mouse mAb (3B10) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-ETS Variant 6
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~55-60 kDa ETV6 protein.
      BackgroundETV6 contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that this protein is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.
      HostMouse
      ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
      Clone3B10
      IsotypeIgG₁
      Specieshuman
      Positive controlJurkat cells, Human placenta tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review