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MABS827 Anti-ERK1/2 Antibody, clone 16A6.1

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MABS827
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS827
      Replaces05-1152
      DescriptionAnti-ERK1/2 Antibody, clone 16A6.1
      Alternate Names
      • EC: 2.7.11.24
      • MAP kinase 3
      • MAPK3
      • ERT2
      • ERK-1
      • Insulin-stimulated MAP2 kinase
      • MAP kinase isoform p44
      • p44-MAPK
      • MNK1
      • Microtubule-associated protein 2 kinase
      • p44-ERK1
      Background InformationMitogen-activated protein kinase 3 (UniProt: P21708; also known as EC: 2.7.11.24, MAP kinase 3, MAPK3, ERT2, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) is encoded by the mapk3 (also known as Erk1, Prkm3) gene (Gene ID: 50689) in rat. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and -Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ERK1/2 using this mouse monoclonal Anti-ERK1/2 Antibody, clone 16A6.1, Cat. No. MABS827. It is used in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected ERK1/2 in human testis, human cerebellum, rat cerebral cortex, and mouse small intestine tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of rat ERK1.
      Clone16A6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 16A6.1 detects both ERK1 and ERK2 in human, mouse, and rat. This clone targets a sequence corresponding to 21 amino acids in the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • mapk3
      • Erk1
      • Prkm3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed; 43.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ERK1/2 in 10 µg of RAW264.7 cell lysate.


      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications