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AP1173 Anti-EPHA4 Mouse mAb (6H7)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M M Monoclonal Antibody

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AP1173-100UG
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      100 μg
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      Description
      OverviewRecognizes the EPHA4 protein in NIH3T3 cells.
      Catalogue NumberAP1173
      Brand Family Calbiochem®
      SynonymsAnti-Ephrin Receptor A4
      Application Data
      Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlNIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
      Clone6H7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-EPHA4 Mouse mAb (6H7) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Ephrin Receptor A4
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa EPHA4 protein.
      BackgroundThis protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
      Clone6H7
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlNIH3T3 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review