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09-774 Anti-EED Antibody

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100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedPolyclonal Antibody
      Catalogue Number09-774
      DescriptionAnti-EED Antibody
      Alternate Names
      • embryonic ectoderm development
      Background InformationPolycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
      Product Information
      • Jurkat Cell Lysate
      • 3T3/A31 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationUse Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenA synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.
      Epitopea.a. 429-441
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes EED, Mr ~ 62-70 kDa.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, mouse. Predicted to react with rat, oppossum, chicken, dog, and cow based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • HEED
      • WAIT-1
      • WAIT1
      • hEED
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFunction: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates Lys9 and Lys27 of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
      Subunit structure: Interacts with MLL. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with the MA protein of HIV-1.
      Subcellular location: Nucleus. Note: Transiently colocalizes with XIST at inactive X chromosomes.
      Tissue specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
      Molecular Weight~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: 1 μg/mL of this lot detected EED on 10 μg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-EED Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-EED Antibody Certificates of Analysis

      TitleLot Number
      Anti-EED 3077378
      Anti-EED - 2118994 2118994
      Anti-EED - 2391112 2391112
      Anti-EED - 2463626 2463626
      Anti-EED - 2044306 2044306
      Anti-EED - 2194094 2194094
      Anti-EED - 2262722 2262722
      Anti-EED - 2828575 2828575
      Anti-EED - 3255444 3255444
      Anti-EED - 3543913 3543913


      Reference overviewApplicationSpeciesPub Med ID
      The central role of EED in the orchestration of polycomb group complexes.
      Cao, Q; Wang, X; Zhao, M; Yang, R; Malik, R; Qiao, Y; Poliakov, A; Yocum, AK; Li, Y; Chen, W; Cao, X; Jiang, X; Dahiya, A; Harris, C; Feng, FY; Kalantry, S; Qin, ZS; Dhanasekaran, SM; Chinnaiyan, AM
      Nature communications  5  3127  2014

      Show Abstract
      Immunoprecipitation24457600 24457600
      Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.
      De Cegli, R; Iacobacci, S; Flore, G; Gambardella, G; Mao, L; Cutillo, L; Lauria, M; Klose, J; Illingworth, E; Banfi, S; di Bernardo, D
      Nucleic acids research  41  711-26  2013

      Show Abstract
      Mouse23180766 23180766
      Epigenetic therapy with 3-deazaneplanocin A, an inhibitor of the histone methyltransferase EZH2, inhibits growth of non-small cell lung cancer cells.
      Junko Kikuchi,Taichi Takashina,Ichiro Kinoshita,Eiki Kikuchi,Yasushi Shimizu,Jun Sakakibara-Konishi,Satoshi Oizumi,Victor E Marquez,Masaharu Nishimura,Hirotoshi Dosaka-Akita
      Lung cancer (Amsterdam, Netherlands)  78  2012

      Show Abstract
      22925699 22925699
      Ezh2 expression in astrocytes induces their dedifferentiation toward neural stem cells.
      Sher, Falak, et al.
      Cell Reprogram, 13: 1-6 (2011)  2011

      Show Abstract
      20979531 20979531
      Synergy of Eed and Tsix in the repression of Xist gene and X-chromosome inactivation.
      Shibata, S; Yokota, T; Wutz, A
      The EMBO journal  27  1816-26  2008

      Show Abstract Full Text Article
      Western Blotting18511907 18511907
      The murine polycomb group protein Eed is required for global histone H3 lysine-27 methylation.
      Montgomery, Nathan D, et al.
      Curr. Biol., 15: 942-7 (2005)  2005

      Show Abstract
      15916951 15916951
      Different EZH2-containing complexes target methylation of histone H1 or nucleosomal histone H3.
      Kuzmichev, Andrei, et al.
      Mol. Cell, 14: 183-93 (2004)  2004

      Show Abstract
      15099518 15099518
      Transcriptional repression mediated by the human polycomb-group protein EED involves histone deacetylation.
      van der Vlag, J and Otte, A P
      Nat. Genet., 23: 474-8 (1999)  1999

      Show Abstract
      10581039 10581039

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      Life Science Research > Antibodies and Assays > Primary Antibodies