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07-1364 Anti-ECT2 Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-1364
      DescriptionAnti-ECT2 Antibody
      Alternate Names
      • epithelial cell transforming sequence 2 oncogene
      • Epithelial cell-transforming sequence 2 oncogene
      • protein ECT2
      Background InformationProtein ECT2 (UniProt: Q9H8V3; also known as Epithelial cell-transforming sequence 2 oncogene) is encoded by the ECT2 gene (Gene ID: 1894) in human. ECT2 is a Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP and promotes guanine nucleotide exchange on the Rho family members of small GTPases. It is expressed in lung epithelial cells. Its expression is also observed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. Its levels are up-regulated by calcium in cells forming cell-cell contact sites. It is also up-regulated by DNA damaging agents like hydrogen peroxide and ionizing radiation. ECT2 is required for signal transduction pathways involved in the regulation of cytokinesis. It plays a role in the control of mitotic spindle assembly and regulates the activation of Cdc42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. It contains two BCRT domains (aa 171-260 and 266-354) and the second BCRT domain is involved in inhibition, probably by helping to hamper RhoA binding. ECT2 is autoinhibited by the C-terminal PH domain (aa 675-794) that folds back and binds to the surface of the DH domain (aa 452-641), blocking binding of RhoA to the catalytic center of the DH domain. ECT2 is phosphorylated by PLK1 in vitro and is hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at threonine 373 occurs during the G2/M phase that relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at threonine 444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. (Ref.: Chen, M., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(2); 1027-1035; Kim, JE., et al. (2005). J. Biol. Chem. 280(7); 5733-5739; Tatsumoto, T., et al. (1999). J. Cell Biol. 147(5); 921-928).
      Product Information
      FormatAffinity Purified
      • A549 cell lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      ApplicationAnti-ECT2, Cat. No. 07-1364, is a rabbit polyclonal antibody that detects ECT2 protein and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Protein ECT2 in A549 cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human ECT2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis rabbit polyclonal antibody detects ECT2. It targets an epitope within 15 amino acids from the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Monkey, Bovine, Opossum, Porcine, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARHGEF31
      • FLJ10461
      • MGC138291
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Binds highly specifically to RhoA, RhoC and Rac proteins, but does not appear to catalyze guanine nucleotide exchange By similarity.
      SUBUNIT STRUCTURE: Associates with RACGAP1 at anaphase and during cytokinesis.
      SEQUENCE SIMILARITES: Contains 2 BRCT domains.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      CAUTION: It is uncertain whether Met-1 or Met-146 is the initiator.
      Molecular Weight~105 kDa observed; 103.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Protein ECT2 in A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      07-1364 04053252413094


      Anti-ECT2 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-ECT2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-ECT2 - 2461556 2461556
      Anti-ECT2 - 1946562 1946562
      Anti-ECT2 - 2051415 2051415
      Anti-ECT2 - 2201502 2201502
      Anti-ECT2 - 2490687 2490687
      Anti-ECT2 - 3041056 3041056
      Anti-ECT2 - 3218760 3218760
      Anti-ECT2 - 3290436 3290436
      Anti-ECT2 - 3447386 3447386
      Anti-ECT2 - 3761835 3761835


      Reference overviewPub Med ID
      MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly.
      Guillemot, L; Guerrera, D; Spadaro, D; Tapia, R; Jond, L; Citi, S
      Molecular biology of the cell  25  1995-2005  2014

      Show Abstract
      24807907 24807907