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MABF2248-100UG Anti-EBV LMP1 Antibody, clone S12

MABF2248-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2248-100UG
      DescriptionAnti-EBV LMP1 Antibody, clone S12
      Alternate Names
      • Latent membrane protein
      • p63
      Background InformationLatent membrane protein 1 (UniProt: P03230; also known as LMP-1, p63) Is encoded by the LMP1 (also known as BNLF1) gene (Gene ID: 3783750) in Epstein-Barr virus. Epstein-Barr virus (EBV) is shown to the causative agent of infectious mononucleosis and it has been associated with human malignancies, such as the African Burkitt's lymphoma and nasopharyngeal carcinoma. EBV is reported to have a unique tropism for resting B lymphocytes and causes them to transform into continuously proliferating lymphoblasts. Latent Membrane Protein 1 (LMP-1) is the dominant oncogene expressed by EBV. It is expressed in the plasma membrane of transformed B cells latently infected with the virus. It is not found in uninfected B cells. It is a type III multi-pass integral membrane protein with short hydrophilic N terminus, six hydrophobic membrane spanning regions, and a relatively long C-terminal cytoplasmic tail. It is only found in cells with EBV genetic information and plays a role in the EBV-induced B-cell transformation and immortalization. It acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. It acts as a constitutively activated receptor of the tumor necrosis factor (TNF) receptor superfamily and activates mitogen-activated kinases, NF-kB, and the JAK/STAT pathway via TNF receptor-associated factors (TRAFs). (Ref.: Mann, KP., et al. (1985). J. Virology 55(3); 710-720; Xu, J., et al. (2002). J. Viology. 76(8); 4080-4086).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-EBV LMP1, clone S12 , Cat. No. MABF2248, is a mouse monoclonal antibody that detects Latent membrane protein 1 in EBV-transfomed cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immuohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected EBV LMP1 in Immunocytochemistry applications (Xu, J., et. al. (2002). J Virol. 76(8):4080-6).

      Flow Cytometry Analysis: A representative lot detected EBV LMP1 in Flow Cytometry applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

      Western Blotting Analysis: A representative lot detected EBV LMP1 in Western Blotting applications (Choi, I.K., et. al. (2018). Proc Natl Acad Sci USA. 115(4):E686-E695; Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65; Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

      Immunofluorescence Analysis: A representative lot detected EBV LMP1 in Immunofluorescence applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

      Immunohistochemistry Analysis: A representative lot detected EBV LMP1 in Immunohistochemistry applications (Brink, A.A., et. al. (1997). J Clin Pathol. 50(11):911-8; Khabir, A., et. al. (2005). Virol J. 2:39).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated EBV LMP1 in Immunoprecipitation applications (Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65).
      Biological Information
      ImmunogenA fusion protein (pKH548) consisting of 189 amino acids from the C-terminus region of LMP1 coupled to beta-galactosidase.
      CloneS12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone S12 is a mouse monoclonal antibody that specifically detects Latent membrane protein 1 (LMP1) in Epstein-Barr virus transformed cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus transformed human cells.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LMP1
      • BNLF1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~59 kDa observed; 41.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected LMP1 in lysates from Raji cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-EBV LMP1 Antibody, clone S12 Certificates of Analysis

      TitleLot Number
      Anti-EBV LMP1, clone S12 - 34939963493996
      Anti-EBV LMP1, clone S12 - Q3224768Q3224768

      References

      Reference overviewPub Med ID
      Signaling by the Epstein-Barr virus LMP1 protein induces potent cytotoxic CD4+ and CD8+ T cell responses.
      Choi, IK; Wang, Z; Ke, Q; Hong, M; Qian, Y; Zhao, X; Liu, Y; Kim, HJ; Ritz, J; Cantor, H; Rajewsky, K; Wucherpfennig, KW; Zhang, B
      Proc Natl Acad Sci U S A  115  E686-E695  2018

      Show Abstract
      29311309 29311309
      EBV latent membrane protein 1 abundance correlates with patient age but not with metastatic behavior in north African nasopharyngeal carcinomas.
      Khabir, A; Karray, H; Rodriguez, S; Rosé, M; Daoud, J; Frikha, M; Boudawara, T; Middeldorp, J; Jlidi, R; Busson, P
      Virol J  2  39  2005

      Show Abstract
      15842731 15842731
      Preferential localization of the Epstein-Barr virus (EBV) oncoprotein LMP-1 to nuclei in human T cells: implications for its role in the development of EBV genome-positive T-cell lymphomas.
      Xu, J; Ahmad, A; Menezes, J
      J Virol  76  4080-6  2002

      Show Abstract
      11907247 11907247
      Presence of Epstein-Barr virus latency type III at the single cell level in post-transplantation lymphoproliferative disorders and AIDS related lymphomas.
      Brink, AA; Dukers, DF; van den Brule, AJ; Oudejans, JJ; Middeldorp, JM; Meijer, CJ; Jiwa, M
      J Clin Pathol  51  911-9  1998

      Show Abstract
      9462239 9462239
      Epstein-Barr virus gene expression in nasopharyngeal carcinoma.
      Young, LS; Dawson, CW; Clark, D; Rupani, H; Busson, P; Tursz, T; Johnson, A; Rickinson, AB
      J Gen Virol  70 ( Pt 5)  1051-66  1989

      Show Abstract
      2836550 2836550
      Epstein-Barr virus-encoded protein found in plasma membranes of transformed cells.
      Mann, KP; Staunton, D; Thorley-Lawson, DA
      J Virol  56  710-21  1986

      Show Abstract
      2991591 2991591