MABT26 Anti-E-cadherin Antibody, clone DECMA-1

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MABT26
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IHC, ICC, IP, FC, FUNC R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT26
      DescriptionAnti-E-cadherin Antibody, clone DECMA-1
      Alternate Names
      • CAM 120/80
      • CD324 antigen
      • Cadherin 1, E-cadherin (epithelial)
      • cadherin 1, type 1
      • cadherin 1, type 1, E-cadherin (epithelial)
      • calcium-dependent adhesion protein, epithelial
      • cell-CAM 120/80
      Background InformationCadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium-binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated, consistent with its function of an invasion suppressor in normal epithelia.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      PresentationPurified rat monoclonal IgG1κ in buffer containing PBS without preservative.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-E-cadherin Antibody, clone DECMA-1 is validated for use in WB, IH, IC, IP, FC, FUNC for the detection of E-cadherin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunoprecipitation
      • Flow Cytometry
      • Affects Function
      Application NotesImmunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect E-cadherin in mouse GI tract tissue.

      Inhibition: A previous lot was used by an independent laboratory in inhibion studies, blocking E-cadherin associated cell adhesion. (Vestweber, 1985)

      Flow Cytometry Analysis: A previous lot was used by an independent laboratory in flow cytometry applications. (Tang, 1994)
      Biological Information
      ImmunogenMouse embryonal carcinoma cell line PCC4
      CloneDecma-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Arc-1
      • CD324
      • CDHE
      • Cadherin-1
      • E-cadherin
      • ECAD
      • LCAM
      • UVO
      • Uvomorulin
      • uvomorulin
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (by similarity).

      E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (by similarity).

      SUBUNIT STRUCTURE: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5 (by similarity).

      SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm (by similarity).

      TISSUE SPECIFICTY: Non-neural epithelial tissues.

      DEVELOPMENTAL STAGE: In the testis, expression is highest in fetal gonad, then decreases 5-fold in newborn. Detectable in 7-day-old but not in 21-day-old or adult.

      SEQUENCE SIMILARITIES: Contains 5 cadherin domains.
      Molecular Weight~ 106 kDa observed MW.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-E-cadherin Antibody, clone DECMA-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-E-cadherin Antibody, clone DECMA-1 Certificates of Analysis

      TitleLot Number
      Anti-E-cadherin, clone DECMA-13167066
      Anti-E-cadherin, clone DECMA-1 - 23911772391177
      Anti-E-cadherin, clone DECMA-1 - 24596522459652
      Anti-E-cadherin, clone DECMA-1 - 19645351964535
      Anti-E-cadherin, clone DECMA-1 - 19962471996247
      Anti-E-cadherin, clone DECMA-1 - 20264172026417
      Anti-E-cadherin, clone DECMA-1 - 20554762055476
      Anti-E-cadherin, clone DECMA-1 - 21671882167188
      Anti-E-cadherin, clone DECMA-1 - 22756482275648
      Anti-E-cadherin, clone DECMA-1 - 23306222330622

      References

      Reference overviewPub Med ID
      TRPM8 on mucosal sensory nerves regulates colitogenic responses by innate immune cells via CGRP.
      de Jong, PR; Takahashi, N; Peiris, M; Bertin, S; Lee, J; Gareau, MG; Paniagua, A; Harris, AR; Herdman, DS; Corr, M; Blackshaw, LA; Raz, E
      Mucosal immunology  8  491-504  2015

      Show Abstract
      25269705 25269705
      Improving outcomes of acute kidney injury using mouse renal progenitor cells alone or in combination with erythropoietin or suramin.
      Han, X; Zhao, L; Lu, G; Ge, J; Zhao, Y; Zu, S; Yuan, M; Liu, Y; Kong, F; Xiao, Z; Zhao, S
      Stem cell research & therapy  4  74  2013

      Show Abstract
      23777889 23777889
      The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion.
      Comijn, J, et al.
      Mol. Cell, 7: 1267-78 (2001)  2001

      Show Abstract
      11430829 11430829
      Inhibition of invasion of epithelial cells by Tiam1-Rac signaling.
      Hordijk, P L, et al.
      Science, 278: 1464-6 (1997)  1997

      Show Abstract
      9367959 9367959
      A targeted mutation in the mouse E-cadherin gene results in defective preimplantation development.
      Riethmacher, D, et al.
      Proc. Natl. Acad. Sci. U.S.A., 92: 855-9 (1995)  1995

      Show Abstract
      7846066 7846066
      E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro.
      Tang, A, et al.
      J. Cell. Sci., 107 ( Pt 4): 983-92 (1994)  1994

      Show Abstract
      8056851 8056851

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