05-298 Anti-α-Dystroglycan Antibody, clone VIA4-1

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, Gp, H, M, R, Rb IHC, WB M Culture Supernatant Monoclonal Antibody
      Catalogue Number05-298
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-α-Dystroglycan Antibody, clone VIA4-1
      OverviewNote: This product may be used for research purposes only. Diagnostic use of this product requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.
      Background InformationDystroglycan is a component of the dystrophin glycoprotein complex that serves as an adhesion molecule and links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-29) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 653-654) to produce alpha-dystroglycan (aa 30-653) and beta-dystroglycan (aa 654-895). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand- binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).
      Product Information
      FormatCulture Supernatant
      • Mouse, rat and human skeletal muscle tissue extracts or DU 145 (human prostate carcinoma tumor) cell lysate
      PresentationCulture supernatant containing 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect α-Dystroglycan using this Anti-α-Dystroglycan Antibody, clone VIA4-1. This antibody has been published and validated for use in IH and WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      ImmunogenRabbit skeletal muscle membrane preparation
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes α-dystroglycan/LARGE-glycan.
      Species Reactivity
      • Canine
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
      Gene Symbol
      • DAG1
      • A3a
      • DAG
      • beta-dystroglycan
      • alpha-dystroglycan
      • AGRNR
      • 156DAG
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14118 # Forms part of the dystrophin-associated protein complex (DAPC) which may link the cytoskeleton to the extracellular matrix. Alpha-dystroglycan functions as a laminin receptor. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells.
      SIZE: 895 amino acids; 97581 Da
      SUBUNIT: Interacts with SGCD (By similarity). Interacts with AGR2 and AGR3.
      SUBCELLULAR LOCATION: Alpha-dystroglycan: Secreted, extracellular space. & Beta-dystroglycan: Cell membrane; Single- pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed in a variety of fetal and adult tissues.
      Molecular Weight156 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on rabbit skeletal muscle in a western blot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information


      Anti-α-Dystroglycan Antibody, clone VIA4-1 SDS


      Safety Data Sheet (SDS) 

      Anti-α-Dystroglycan Antibody, clone VIA4-1 Certificates of Analysis

      TitleLot Number
      Anti-#945;-Dystroglycan, clone VIA4-1 - 21501582150158
      Anti-#945;-Dystroglycan, clone VIA4-1 - DAM1834934DAM1834934
      Anti--Dystroglycan, clone VIA4-1 - 19519741951974
      Anti--Dystroglycan, clone VIA4-1 - 19938961993896
      Anti--Dystroglycan, clone VIA4-1 - 20895092089509
      Anti--Dystroglycan, clone VIA4-1 - DAM1408802DAM1408802
      Anti--Dystroglycan, clone VIA4-1 - DAM1632063DAM1632063
      Anti--Dystroglycan, clone VIA4-1 - DAM1764385DAM1764385
      Anti--Dystroglycan, clone VIA4-1 - DAM179797926DAM179797926
      Anti--Dystroglycan, clone VIA4-1 - JBC1863401JBC1863401


      Reference overviewApplicationSpeciesPub Med ID
      Absence of glial α-dystrobrevin causes abnormalities of the blood-brain barrier and progressive brain edema.
      Lien, CF; Mohanta, SK; Frontczak-Baniewicz, M; Swinny, JD; Zablocka, B; Górecki, DC
      The Journal of biological chemistry  287  41374-85  2012

      Show Abstract
      23043099 23043099
      Quiescence and activation of stem and precursor cell populations in the subependymal zone of the mammalian brain are associated with distinct cellular and extracellular matrix signals.
      Kazanis, Ilias, et al.
      J. Neurosci., 30: 9771-81 (2010)  2010

      Show Abstract
      20660259 20660259
      Synapse formation and clustering of neuroligin-2 in the absence of GABAA receptors.
      Patrizi, Annarita, et al.
      Proc. Natl. Acad. Sci. U.S.A., 105: 13151-6 (2008)  2008

      Show Abstract
      ImmunofluorescenceMouse18723687 18723687
      Worldwide distribution and broader clinical spectrum of muscle-eye-brain disease.
      Taniguchi, Kiyomi, et al.
      Hum. Mol. Genet., 12: 527-34 (2003)  2003

      Show Abstract
      Immunohistochemistry (Tissue)12588800 12588800
      Mechanistic distinctions between agrin and laminin-1 induced aggregation of acetylcholine receptors.
      Lee, LK; Kunkel, DD; Stollberg, J
      BMC neuroscience  3  10  2002

      Show Abstract
      12182759 12182759
      Dystroglycan is present in rat thyroid and rat thyroid cells and responds to thyrotropin.
      Collins, B J, et al.
      Endocrinology, 142: 3152-62 (2001)  2001

      Show Abstract
      Immunocytochemistry, Immunoblotting (Western)11416038 11416038
      A stoichiometric complex of neurexins and dystroglycan in brain
      Sugita, S., et al
      J Cell Biol, 154:435-45 (2001)  2001

      Immunocytochemistry, Immunoblotting (Western)11470830 11470830
      Dystrophic phenotype induced in vitro by antibody blockade of muscle alpha-dystroglycan-laminin interaction
      Brown, S. C., et al
      J Cell Sci, 112 ( Pt 2):209-16 (1999)  1999

      9858474 9858474
      MEK kinase 1, a substrate for DEVD-directed caspases, is involved in genotoxin-induced apoptosis.
      Widmann, C, et al.
      Mol. Cell. Biol., 18: 2416-29 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9528810 9528810
      Membrane organization of the dystrophin-glycoprotein complex.
      Ervasti, J M and Campbell, K P
      Cell, 66: 1121-31 (1991)  1991

      Show Abstract
      1913804 1913804

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