324405 Anti-Dopamine D₄ Receptor Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Pricing & Availability

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324405-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
      Catalogue Number324405
      Brand Family Calbiochem®
      References
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1:500)
      Immunohistochemistry (1:10,000)
      Application CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Dopamine D₄ Receptor Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dopamine D₄ Receptor Rabbit pAb Certificates of Analysis

      TitleLot Number
      324405

      References

      Reference overview
      Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:500)
      Immunohistochemistry (1:10,000)
      DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
      IsotypeIgG
      Specieshuman, rat
      Positive controlRat brain
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
      Toxicity Standard Handling
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.