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MAB427 Anti-Dihydropyridine-sensitive Calcium Channel α 1 Subunit Antibody, clone 1a

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MAB427
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbFC, IP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB427
      Replaces06-422
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Dihydropyridine-sensitive Calcium Channel α 1 Subunit Antibody, clone 1a
      References
      Product Information
      FormatAscites
      PresentationAscites.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Dihydropyridine-sensitive Calcium Channel α 1 Subunit Antibody, clone 1a is an antibody against Dihydropyridine-sensitive Calcium Channel α 1 Subunit for use in FC, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot, immunoprecipitation and FACS.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ClonemAB 1a
      HostMouse
      SpecificityDihydropyridine calcium channel, a1 subunit as determined by Western blot (~210 kDa), immunoprecipitation and functional test. Membrane preparations are recommended. The mouse muscle cell line, BC3H1 functions as a good positive control cell line. Reactive with L-type calcium channel alpha1 subunit. Specific for small cell carcinoma and neuroblastoma by FACS analysis. No interaction with squamous cell carcinoma or with cardiac calcium chanel isoforms.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility.
      Gene Symbol
      • CACNA1S
      • CACH1
      • CCHL1A3
      • hypoPP
      • MHS5
      • CACNL1A3
      • Cav1.1
      • HOKPP
      • CACN1
      • HypoPP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13698 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.
      SIZE: 1873 amino acids; 212164 Da
      SUBUNIT: Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. An additional gamma subunit is present only in skeletal muscle L-type channel.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Skeletal muscle specific.
      DOMAIN: SwissProt: Q13698 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. & The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.
      PTM: Phosphorylation by PKA activates the calcium channel (By similarity).
      DISEASE: SwissProt: Q13698 # Defects in CACNA1S are a cause of hypokalemic periodic paralysis (HYPOKPP) [MIM:170400]; also designated HOKPP. HYPOKPP is an autosomal dominant skeletal muscle disorder manifested by episodic weakness associated with low serum potassium. Muscle weakness could be due to altered excitation-contraction coupling in HYPOKPP patients. & Defects in CACNA1S are the cause of malignant hyperthermia susceptibility 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
      SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB427 04053252318108

      Documentation

      Anti-Dihydropyridine-sensitive Calcium Channel α 1 Subunit Antibody, clone 1a SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dihydropyridine-sensitive Calcium Channel α 1 Subunit Antibody, clone 1a Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (#945;1-Subunit) MONOCLONAL ANTIBODY - 2140030 2140030
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (a1-Subunit) MONOCLONAL ANTIBODY 2967228
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (a1-Subunit) MONOCLONAL ANTIBODY - 1999447 1999447
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (a1-Subunit) MONOCLONAL ANTIBODY - 2055971 2055971
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (a1-Subunit) MONOCLONAL ANTIBODY - 2092683 2092683
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (a1-Subunit) MONOCLONAL ANTIBODY - 2274007 2274007
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (α1-Subunit) - 3398053 3398053
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (α1-Subunit) - 3597284 3597284
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (α1-Subunit) - 3901435 3901435
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (α1-Subunit) - 3995168 3995168

      References

      Reference overviewPub Med ID
      Localization of Cacna1s to ON bipolar dendritic tips requires mGluR6-related cascade elements.
      Tummala, SR; Neinstein, A; Fina, ME; Dhingra, A; Vardi, N
      Investigative ophthalmology & visual science  55  1483-92  2014

      Show Abstract
      24519419 24519419
      Increased muscle stress-sensitivity induced by selenoprotein N inactivation in mouse: a mammalian model for SEPN1-related myopathy.
      Rederstorff, M; Castets, P; Arbogast, S; Lainé, J; Vassilopoulos, S; Beuvin, M; Dubourg, O; Vignaud, A; Ferry, A; Krol, A; Allamand, V; Guicheney, P; Ferreiro, A; Lescure, A
      PloS one  6  e23094  2011

      Show Abstract
      21858002 21858002
      Caveolin-3 is a direct molecular partner of the Cav1.1 subunit of the skeletal muscle L-type calcium channel.
      Harold Couchoux,Hicham Bichraoui,Christophe Chouabe,Xavier Altafaj,Robert Bonvallet,Bruno Allard,Michel Ronjat,Christine Berthier
      The international journal of biochemistry & cell biology  43  2011

      Show Abstract
      21262376 21262376
      Trophic action of sphingosine 1-phosphate in denervated rat soleus muscle.
      Marika Zanin,Elena Germinario,Luciano Dalla Libera,Dorianna Sandonà,Roger A Sabbadini,Romeo Betto,Daniela Danieli-Betto
      American journal of physiology. Cell physiology  294  2008

      Show Abstract
      17942639 17942639
      Isoform switching in myofibrillar and excitation-contraction coupling proteins contributes to diminished contractile function in regenerating rat soleus muscle.
      Esposito, A; Germinario, E; Zanin, M; Palade, PT; Betto, R; Danieli-Betto, D
      Journal of applied physiology (Bethesda, Md. : 1985)  102  1640-8  2007

      Show Abstract
      17234797 17234797
      Loss of caveolin-3 induced by the dystrophy-associated P104L mutation impairs L-type calcium channel function in mouse skeletal muscle cells.
      Couchoux, H; Allard, B; Legrand, C; Jacquemond, V; Berthier, C
      The Journal of physiology  580  745-54  2007

      Show Abstract Full Text Article
      17317753 17317753
      Properties and role of voltage-dependent calcium channels during mouse skeletal muscle differentiation.
      Isabelle Bidaud, Arnaud Monteil, Joël Nargeot, Philippe Lory
      Journal of muscle research and cell motility  27  75-81  2006

      Show Abstract
      16538437 16538437
      The dynamic range and domain-specific signals of intracellular calcium in photoreceptors.
      Szikra, T; Krizaj, D
      Neuroscience  141  143-55  2006

      Show Abstract
      16682126 16682126
      Calcium channels are involved in calcium oxalate crystal formation in specialized cells of Pistia stratiotes L.
      Volk, GM; Goss, LJ; Franceschi, VR
      Annals of botany  93  741-53  2004

      Show Abstract
      15087302 15087302
      Modulation of L-type calcium channel expression during retinoic acid-induced differentiation of H9C2 cardiac cells.
      C Ménard, S Pupier, D Mornet, M Kitzmann, J Nargeot, P Lory
      The Journal of biological chemistry  274  29063-70  1999

      Show Abstract
      10506158 10506158

      Data Sheet

      Title
      MOUSE ANTI-DIHYDROPYRIDINE BINDING COMPLEX (α1-Subunit)