MABT118 Anti-Desmoglein-1 Antibody, clone 32-2B

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MABT118
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IF, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT118
      DescriptionAnti-Desmoglein-1 Antibody, clone 32-2B
      Alternate Names
      • Desmoglein-1
      • Desmosomal glycoprotein 1
      • DG1
      • DGI
      • Pemphigus foliaceus antigen
      Background InformationDesmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. The desmoglein-1 subtype is highly expressed in the epidermis. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesions. Desmoglein-1 is regulated by calcium and PKCα signaling, and is also coupled to intermediate filaments of the cytoskeleton. Desmosomes play a critical role in cell and tissue stability, and may contribute to the progression of cancer.
      References
      Product Information
      FormatPurified
      Control
      • Human tonsil tissue lysate.
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Desmoglein-1, clone 32-2B detects levels of Desmoglein-1 proteins & has been published & validated for use in WB, IF, IHC & ICC.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

      Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

      Western Blot Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in bovine epidermal desmosomal cores and in human keratinocyte cell lysates. (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

      Immunofluorescent Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in MDCK cells (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in human epidermal tissue (Maruani, A., et al. (2008). Am J Clin Pathol. 130(3):369-374.).
      Biological Information
      ImmunogenBovine Desmoglein-1 from nasal epidermis
      Clone32-2B
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DSG1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein by similarity. Cell junction > desmosome.

      TISSUE SPECIFICTY: Epidermis, muzzle, tongue and esophagus.

      DOMAIN: Calcium may be bound by the cadherin-like repeats.

      SEQUENCE SIMILARITIES: Contains 4 cadherin domains.
      Molecular Weight~150 kDa observed. This protein may be glycosolated. Uniprot describes a molecular weight at ~112 kDa; however, Desmoglein-1 has been observed at ~150 kDa (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human tonsil tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Desmoglein-1 in 10 µg of human tonsil tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Desmoglein-1 Antibody, clone 32-2B SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Desmoglein-1 Antibody, clone 32-2B Certificates of Analysis

      TitleLot Number
      Anti-Desmoglein-1, clone 32-2B - 23911732391173
      Anti-Desmoglein-1, clone 32-2B - 29861392986139
      Anti-Desmoglein-1, clone 32-2B - 31850233185023
      Anti-Desmoglein-1, clone 32-2B - 34514233451423
      Anti-Desmoglein-1, clone 32-2B - Q2006986Q2006986
      Anti-Desmoglein-1, clone 32-2B -25901662590166
      Anti-Desmoglein-1, clone 32-2B Monoclonal Antibody2967245
      Anti-Desmoglein-1, clone 32-2B Monoclonal Antibody3054702

      References

      Reference overviewPub Med ID
      Immunostaining with antibodies to desmoglein provides the diagnosis of drug-induced pemphigus and allows prediction of outcome.
      Maruani, Annabel, et al.
      Am. J. Clin. Pathol., 130: 369-74 (2008)  2008

      Show Abstract
      18701409 18701409
      A simple epithelial cell line (MDCK) shows heterogeneity of desmoglein isoforms, one resembling pemphigus vulgaris antigen.
      Vilela, M J, et al.
      J. Cell. Sci., 108 ( Pt 4): 1743-50 (1995)  1995

      Show Abstract
      7615689 7615689
      Immunohistochemical detection of desmosomes in oral squamous cell carcinomas: correlation with differentiation, mode of invasion, and metastatic potential.
      Harada, T, et al.
      Int J Oral Maxillofac Surg, 21: 346-9 (1992)  1992

      Show Abstract
      1282923 1282923
      Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas.
      Conn, I G, et al.
      Br J Urol, 65: 176-80 (1990)  1990

      Show Abstract
      2317652 2317652
      Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology.
      Vilela, M J, et al.
      J. Pathol., 153: 365-75 (1987)  1987

      Show Abstract
      3323436 3323436

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies