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MABN2284 Anti-Delta-like protein 1 Antibody, clone PGPM-1F9

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MABN2284
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, WB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2284
      DescriptionAnti-Delta-like protein 1 Antibody, clone PGPM-1F9
      Alternate Names
      • Drosophila Delta homolog 1
      • Delta1
      Background InformationDelta-like protein 1 (UniProt: Q61483; also known as Drosophila Delta homolog 1, Delta1, DLL1) is encoded by the Dll1 gene (Gene ID: 13388) in murine species. DLL1 is a single-pass transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner. Following transinteraction, ligand cells produce mechanical force that promotes Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling. Delta1 is required for embryonic development and maintenance of adult stem cells in many different tissues. DLL1-induced Notch signaling plays a role in the regulation of cell lineage, cell specification, cell patterning, and morphogenesis through effects on differentiation and proliferation. DLL1 is reported to play a role in regulating neuronal differentiation of neural precursor cells via cell-cell interaction. DLL1 is reported to be co-expressed with DLL3 in the presomtic mesoderm of mouse embryo, however despite their co-expression, mutations in these proteins cause strikingly different effects. Heterozygous DLL1 mutant mice are lighter and smaller, with altered fat to lean ratio and display increased blood pressure and a slight bradycardia. They also exhibit reduced cholesterol and triglyceride levels in blood.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Delta-like protein 1 antibody, clone PGPM-1F9, Cat. No. MABN2284, is a highly specific rat monoclonal antibody that targets Delta-like protein 1 and has been tested in Flow Cytometry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected Delta-like protein 1 in Immunohistochemistry applications (Bone, R.A., et. al. (2014). Development. 141(24):4806-16).

      Flow Cytometry Analysis: 1 µg from a representative lot detected Delta-like protein 1 in one million DLL1-flag A7A1/CHO cells expressing DLL1.

      Western Blotting Analysis: A representative lot detected Delta-like protein 1 in Western Blotting applications (Geffers, I., et. al. (2007). J Cell Biol. 178(3):465-76).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of murine Delta-like protein 1.
      ClonePGPM-1F9
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone PGPM-1F9 specifically detects murine Delta Like protein 1. It targets an epitope within 17 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dll1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~78 kDa observed; 78.45 calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DLL1-FLAG/CHO cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Delta-like protein 1 in 10 µg of lysate from CHO cells stably expressing Flag-tagged Delta-like Protein 1.


      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications