AB16961 | Anti-DFF45/35 Antibody, NT

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB Rb Purified Polyclonal Antibody
      Catalogue NumberAB16961
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DFF45/35 Antibody, NT
      Product Information
      PresentationAffinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
      ApplicationThis Anti-DFF45/35 Antibody, N-terminus is validated for use in IP, WB for the detection of DFF45/35.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1:500-1:1000


      Whole cell lysate from HeLa cells can be used as a positive control and a 45 kDa band should be detected.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPolyclonal antibody raised against a peptide corresponding to amino acids 2-21 at N-terminus of human DFF (Liu et al. 1997).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause DNase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryApoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • DFFA
      • DFF45
      • DFF-45
      • ICAD
      • DFF1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00273 # Inhibitor of the caspase-activated DNase (DFF40).
      SIZE: 331 amino acids; 36522 Da
      SUBUNIT: Heterodimer of DFFA and DFFB.
      PTM: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: O00273 ## Contains 1 CIDE-N domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-DFF (N-Terminal) POLYCLONAL ANTIBODY - 2362839 2362839


      Reference overviewPub Med ID
      Apoptosis. An endonuclease at last.
      Wyllie, A
      Nature, 391: 20-1 (1998)  1998

      9422500 9422500
      Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.
      Sakahira, H, et al.
      Nature, 391: 96-9 (1998)  1998

      Show Abstract
      9422513 9422513
      Involvement of an ICE-like protease in Fas-mediated apoptosis.
      Enari, M, et al.
      Nature, 375: 78-81 (1995)  1995

      Show Abstract
      7536900 7536900

      Data Sheet

      Anti-DFF45/35, N-terminus - Data Sheet
      RABBIT ANTI-DFF (N-Terminal)

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      Life Science Research > Antibodies and Assays > Primary Antibodies