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AB1248 Anti-Cytochrome P450 Enzyme CYP1A2 Antibody

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AB1248
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1248
      ReplacesMAB3526
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytochrome P450 Enzyme CYP1A2 Antibody
      References
      Product Information
      FormatSerum
      PresentationRabbit antiserum. Liquid. No preservatives
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytochrome P450 Enzyme CYP1A2 Antibody is an antibody against Cytochrome P450 Enzyme CYP1A2 for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:4,000

      Immunohistochemistry on frozen and formaldehyde treated sections.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide
      HostRabbit
      SpecificityReacts with human cytochrome P450 enzyme CYP1A2 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes. By Western blot the antibody recognizes a protein with a molecular weight of approximately 54 kDa.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
      Gene Symbol
      • CYP1A2
      • CP12
      • P450(PA)
      • CYPIA2
      • P
      • 350)
      • P3-450
      • P450-P3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05177 # Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen.
      SIZE: 515 amino acids; 58294 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
      TISSUE SPECIFICITY: Liver.
      SIMILARITY: SwissProt: P05177 ## Belongs to the cytochrome P450 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Cytochrome P450 Enzyme CYP1A2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytochrome P450 Enzyme CYP1A2 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HUMAN CYTOCHROME P450 ENZYME CYP1A2 - 2492248 2492248
      RABBIT ANTI-HUMAN CYTOCHROME P450 ENZYME CYP1A2 -2492248A 2492248A
      RABBIT ANTI-HUMAN CYTOCHROME P450 ENZYME CYP1A2 POLYCLONAL ANTIBODY 3015842
      RABBIT ANTI-HUMAN CYTOCHROME P450 ENZYME CYP1A2 POLYCLONAL ANTIBODY - 2277817 2277817

      References

      Reference overviewPub Med ID
      Short synthetic peptides exploited for reliable and specific targeting of antibodies to the C-termini of cytochrome P450 enzymes.
      Edwards, R J, et al.
      Biochem. Pharmacol., 49: 39-47 (1995)  1995

      Show Abstract
      7840781 7840781
      The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature.
      Nelson, D R, et al.
      DNA Cell Biol., 12: 1-51 (1993)  1993

      Show Abstract
      7678494 7678494
      Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes.
      Thomas, P E, et al.
      Fed. Proc., 46: 2563-6 (1987)  1987

      Show Abstract
      3297805 3297805
      P-450 cytochromes: structure and function.
      Black, S D and Coon, M J
      Adv. Enzymol. Relat. Areas Mol. Biol., 60: 35-87 (1987)  1987

      3310532 3310532

      Data Sheet

      Title
      RABBIT ANTI-HUMAN CYTOCHROME P450 ENZYME CYP1A2 POLYCLONAL ANTIBODY