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MABF2262-100UG Anti-Complement receptor 1 Antibody, clone 9H3

MABF2262-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2262-100UG
      DescriptionAnti-Complement receptor 1 Antibody, clone 9H3
      Alternate Names
      • C3b/C4b receptor
      • CD35
      Background InformationComplement receptor type 1 (UniProt: P17927; also known as C3b/C4b receptor, CD35, CR1) is encoded by the CR1 (also known as C3BR) gene (Gene ID: 1378) in human. CR1 is a single-pass type I membrane glycoprotein that is expressed in most peripheral blood cells, follicular-dendritic cells, Schwann cells, and glomerular podocytes. It regulates the activation of the complement cascade by serving as a cofactor for the factor I-mediated cleavage of C3b and C4b and also accelerates the decay of the C3 and C5 convertases. It is shown to bind to C3b and C4b opsonized immune complex on erythrocytes and transports them to liver and spleen where they are degraded by phagocytes. CR1 inhibits spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d. It also plays a role in immune regulation by contributing to the generation of regulatory T cells from activated helper T cells. CR1 is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form with a long extracellular domain (aa 42-1971), a short transmembrane domain (aa 1972-1996) and a cytoplasmic tail (aa 1997-2039). (Ref.: Nickells, M., et al. (1988). Clin. Exp. Immunol. 112(1); 27-33; Reist, CJ et al. (1994). Eur. J. Immunol. 24(9); 2018-2025).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Complement receptor 1, clone 9H3, Cat. No. MABF2262, is a mouse monoclonal antibody that detects complement receptor 1 and has been tested for use in Dot Blot, Flow Cytometry, and Immunocytochemistry.
      Key Applications
      • Dot Blot
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: A representative lot detected Complement receptor 1 in Flow Cytometry applications (Craig, M.L., et. al. (2005). J Immunol. 174(5):3059-66).

      Dot Blot Analysis: A representative lot detected Complement receptor 1 in Dot Blot applications (Nickells, M., et. al. (1998). Clin Exp Immunol. 112(1):27-33).

      Immunocytochemistry Analysis: A representative lot detected Complement receptor 1 in Immunocytochemistry applications (Craig, M.L., et. al. (2005). J Immunol. 174(5):3059-66).
      Biological Information
      ImmunogenSoluble human complement receptor 1
      Epitopeextracellular domain
      Clone9H3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9H3 is a mouse monoclonal antibody that detects human complement receptor type 1. It targets an epitope with in the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Non-Human Primates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CR1
      • C3BR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight223.66 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in TF-1 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor 1 in one million TF-1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Complement receptor 1 Antibody, clone 9H3 Certificates of Analysis

      TitleLot Number
      Anti-Complement receptor 1, clone 9H3 - Q3224767Q3224767

      References

      Reference overviewPub Med ID
      Processing of C3b-opsonized immune complexes bound to non-complement receptor 1 (CR1) sites on red cells: phagocytosis, transfer, and associations with CR1.
      Craig, ML; Waitumbi, JN; Taylor, RP
      J Immunol  174  3059-66  2005

      Show Abstract
      15728520 15728520
      Mapping epitopes for 20 monoclonal antibodies to CR1.
      Nickells, M; Hauhart, R; Krych, M; Subramanian, VB; Geoghegan-Barek, K; Marsh, HC; Atkinson, JP
      Clin Exp Immunol  115  27-36  2001

      Show Abstract
      9566786 9566786