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PC645 Anti-Cleaved Bid (Ab-1) Rabbit pAb

PC645
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M Rb Polyclonal Antibody

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PC645-10T
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      Description
      OverviewRecognizes the ~15 kDa cleaved Bid protein. Does not recognize uncleaved, full-length Bid.
      Catalogue NumberPC645
      Brand Family Calbiochem®
      Application Data
      Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
      Chou, J.J., et al. 1999. Cell 96, 615.
      Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
      Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
      Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
      Luo, X., et al. 1999. Cell 94, 481.
      Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
      Footz, T.K., et al. 1998. Genomics 51, 472.
      Wang, K., et al. 1996. Genes Dev. 10, 2859.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlTNF-α-treated L929 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Application CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Cleaved Bid (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC645

      References

      Reference overview
      Eskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
      Chou, J.J., et al. 1999. Cell 96, 615.
      Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
      Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
      Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
      Luo, X., et al. 1999. Cell 94, 481.
      Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
      Footz, T.K., et al. 1998. Genomics 51, 472.
      Wang, K., et al. 1996. Genes Dev. 10, 2859.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-April-2009 RFH
      ApplicationImmunoblotting (1:1000, chemiluminescence)
      Application Data
      Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa cleaved mouse Bid.
      BackgroundBid (~22 kDa) is a BH3 domain containing pro-apoptotic cytosolic protein that maps to human chromosome 22q11.2. Initially localized to the cytoplasm, the Bid protein is cleaved by the action of caspase 8 into fragments of two different sizes, p15 and p13 and becomes inserted into the mitochondrial membrane. Of the two fragments the p15 BH3 containing fragment is the more potent inducer of apoptosis and in fact is a stronger inducer than the full-length Bid protein. Following cleavage by caspase 8, Bid will bind with Bax localizing it to the mitochondrial membrane and leading to the release of cyto-chrome c. This interaction is blocked by Bcl-x, which directly binds to Bax, competing for the binding of Bax to the membrane-localized Bid.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
      IsotypeIgG
      Speciesnot human, mouse
      Positive controlTNF-α-treated L929 cells
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
      Chou, J.J., et al. 1999. Cell 96, 615.
      Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
      Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
      Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
      Luo, X., et al. 1999. Cell 94, 481.
      Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
      Footz, T.K., et al. 1998. Genomics 51, 472.
      Wang, K., et al. 1996. Genes Dev. 10, 2859.