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MAB319 Anti-Chromogranin A Antibody, clone PHE5

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MAB319
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB319
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Chromogranin A Antibody, clone PHE5
      Alternate Names
      • CgA
      • Pituitary Secretory Protein I
      References
      Product Information
      FormatPurified
      Control
      • POSOTIVE CONTROL: Normal adrenal gland, bowel, or thyroid tissue.
      PresentationLiquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Chromogranin A Antibody, clone PHE5 detects level of Chromogranin A & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:500-1:2,000 on alcohol fixed deparaffinized tissue sections (ABC peroxidase) or 1:10,000 on Methacarn-fixed deparaffinized tissue sections. Will show fine granular cytoplasmic staining associated with secretory granules.

      Western Blot: recognizes a band migrating at approximately 68-75 kDa corresponding to chromogranin A.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman pheochromocytoma
      ClonePHE5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityChromogranin A. The antibody has been extensively documented on immunohistochemical studies in deparaffinized methacarn fixed tissue, where it is a sensitive marker of neuroendocrine cells and tumors. Not recommended for formalin fixed tissues, see MAB5268 or CBL219. On western blots, it identifies the identical bands recognized by antibody LK2H10 (Wilson and Lloyd).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
      Gene Symbol
      • CHGA
      • CgA
      • SP-I
      • CGA
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10645 # Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
      SIZE: 457 amino acids; 50730 Da
      SUBCELLULAR LOCATION: Secreted. Note=Neuroendocrine and endocrine secretory granules.
      PTM: Sulfated on tyrosine residues and/or contains sulfated glycans.
      SIMILARITY: SwissProt: P10645 ## Belongs to the chromogranin/secretogranin protein family.
      MISCELLANEOUS: Binds calcium with a low-affinity.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Chromogranin A Antibody, clone PHE5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Chromogranin A Antibody, clone PHE5 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CHROMOGRANIN A 2485481
      MOUSE ANTI-CHROMOGRANIN A - 3278000 3278000
      MOUSE ANTI-CHROMOGRANIN A -2556288 2556288
      MOUSE ANTI-CHROMOGRANIN A -2835742 2835742
      MOUSE ANTI-CHROMOGRANIN A MONOCLONAL ANTIBODY 2881025
      MOUSE ANTI-CHROMOGRANIN A MONOCLONAL ANTIBODY - 2070822 2070822
      MOUSE ANTI-CHROMOGRANIN A MONOCLONAL ANTIBODY - 2243349 2243349

      References

      Reference overviewPub Med ID
      Amyloid precursor-like protein 1 is differentially upregulated in neuroendocrine tumours of the gastrointestinal tract.
      Arvidsson, Y; Andersson, E; Bergström, A; Andersson, MK; Altiparmak, G; Illerskog, AC; Ahlman, H; Lamazhapova, D; Nilsson, O
      Endocrine-related cancer  15  569-81  2008

      Show Abstract
      18430897 18430897
      Gastrointestinal stromal tumors regularly express synaptic vesicle proteins: evidence of a neuroendocrine phenotype.
      Bümming, P; Nilsson, O; Ahlman, H; Welbencer, A; Andersson, MK; Sjölund, K; Nilsson, B
      Endocrine-related cancer  14  853-63  2007

      Show Abstract
      17914114 17914114

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies