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AB144P Anti-Choline Acetyltransferase Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, Op, Av, Ch, Gp, ZebrafishIH(P), ICC, IHC, WBGtAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB144P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      OverviewCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      Alternate Names
      • Choline acetylase
      • acetyl CoA:choline O-acetyltransferase
      • choline acetyltransferase
      • ChAT
      Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.
      Product Information
      FormatAffinity Purified
      • Human placenta lysates, rat forebrain/rostral hypothalamus.
      PresentationPurified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used at 1:100.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      SpecificityCholine acetyltransferase found in cholinergic neurons in brain and spinal cord.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Opossum
      • Avian
      • Chicken
      • Guinea Pig
      • Zebrafish
      Species Reactivity NoteHuman and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
      Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight70/74 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
      For short term storage (3-4 weeks) maintain at 2-8°C.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information


      Anti-Choline Acetyltransferase Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Choline Acetyltransferase Antibody Certificates of Analysis

      TitleLot Number
      Anti-Choline Acetyltransferase 2464504
      Anti-Choline Acetyltransferase - 2147041 2147041
      Anti-Choline Acetyltransferase - 2383949 2383949
      Anti-Choline Acetyltransferase - 2387396 2387396
      Anti-Choline Acetyltransferase - 2453644 2453644
      Anti-Choline Acetyltransferase - 1978747 1978747
      Anti-Choline Acetyltransferase - 2000801 2000801
      Anti-Choline Acetyltransferase - 2010060 2010060
      Anti-Choline Acetyltransferase - 2041107 2041107
      Anti-Choline Acetyltransferase - 2070392 2070392


      Reference overviewApplicationSpeciesPub Med ID
      Age-Related Changes in Pre- and Postsynaptic Partners of the Cholinergic C-Boutons in Wild-Type and SOD1G93A Lumbar Motoneurons.
      Milan, L; Courtand, G; Cardoit, L; Masmejean, F; Barrière, G; Cazalets, JR; Garret, M; Bertrand, SS
      PloS one  10  e0135525  2015

      Show Abstract
      26305672 26305672
      Vibrissa Self-Motion and Touch Are Reliably Encoded along the Same Somatosensory Pathway from Brainstem through Thalamus.
      Moore, JD; Mercer Lindsay, N; Deschênes, M; Kleinfeld, D
      PLoS biology  13  e1002253  2015

      Show Abstract
      26393890 26393890
      Pharmacogenetic stimulation of cholinergic pedunculopontine neurons reverses motor deficits in a rat model of Parkinson's disease.
      Pienaar, IS; Gartside, SE; Sharma, P; De Paola, V; Gretenkord, S; Withers, D; Elson, JL; Dexter, DT
      Molecular neurodegeneration  10  47  2015

      Show Abstract
      26394842 26394842
      Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems.
      Hua, ZL; Emiliani, FE; Nathans, J
      Neural development  10  21  2015

      Show Abstract
      26395878 26395878
      Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.
      Korzhevskii, DE; Sukhorukova, EG; Kirik, OV; Grigorev, IP
      European journal of histochemistry : EJH  59  2530  2015

      Show Abstract
      26428887 26428887
      Retinal output changes qualitatively with every change in ambient illuminance.
      Tikidji-Hamburyan, A; Reinhard, K; Seitter, H; Hovhannisyan, A; Procyk, CA; Allen, AE; Schenk, M; Lucas, RJ; Münch, TA
      Nature neuroscience  18  66-74  2015

      Show Abstract
      25485757 25485757
      Recording, labeling, and transfection of single neurons in deep brain structures.
      Dempsey, B; Turner, AJ; Le, S; Sun, QJ; Bou Farah, L; Allen, AM; Goodchild, AK; McMullan, S
      Physiological reports  3  2015

      Show Abstract
      25602013 25602013
      Large intercalated neurons of amygdala relay noxious sensory information.
      Bienvenu, TC; Busti, D; Micklem, BR; Mansouri, M; Magill, PJ; Ferraguti, F; Capogna, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  2044-57  2015

      Show Abstract
      25653362 25653362
      Red nucleus and rubrospinal tract disorganization in the absence of Pou4f1.
      Martinez-Lopez, JE; Moreno-Bravo, JA; Madrigal, MP; Martinez, S; Puelles, E
      Frontiers in neuroanatomy  9  8  2015

      Show Abstract
      Immunohistochemistry25698939 25698939
      Pharmacologically distinct nicotinic acetylcholine receptors drive efferent-mediated excitation in calyx-bearing vestibular afferents.
      Holt, JC; Kewin, K; Jordan, PM; Cameron, P; Klapczynski, M; McIntosh, JM; Crooks, PA; Dwoskin, LP; Lysakowski, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3625-43  2015

      Show Abstract
      25716861 25716861


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      Life Science Research > Antibodies and Assays > Primary Antibodies