MABN1780 Anti-Cereblon Antibody, clone 3B12F7

MABN1780
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN1780
      DescriptionAnti-Cereblon Antibody, clone 3B12F7
      Alternate Names
      • Cereblon
      • Protein cereblon
      Background InformationProtein cereblon (UniProt Q96SW2) is encoded by the CRBN (also known as AD-006, MRT2A) gene (Gene ID 51185) in human. Originally identified as a genetic cause of inherited autosomal recessive mental retardation, the human CRBN gene encodes a substrate receptor of a ubiquitin E3 cullin 4-RING ligase (CRL4) complex. A cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (​RBX1) and the adaptor protein damaged ​DNA-binding protein 1 (​DDB1) to form a CRL4 E3 complex, where ​RBX1 serves as the docking site for the activated E2 protein, and ​DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex. Thalidomide and its analogs (lenalidomide, pomalidomide and apremilast) are immunomodulatory drugs (IMiDs) that exert their antiproliferative effects on multiple myeloma (MM) cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation. Human cereblon is a 442-amino acid protein composed of a ​Lon protease-like scaffold (a.a. 80-317) with a helical insertion that mediates ​DDB1 binding and a thalidomide-binding domain (TBD; a.a. 339-442).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect Cereblon using this mouse monoclonal Anti-Cereblon Antibody, clone 3B12F7, Cat. No. MABN1780, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected cereblon in 10 µg of HEK293 cell lysate.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected cereblon in human crebral cortex, kidney, and tonsil tissue sections.


      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human cereblon.
      Clone3B12F7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3B12F7 was raised against an N-terminal sequence present in human cereblon spliced isoforms 1&2 and murine cereblon spliced isoforms 1&3 (UniProt Q96SW2 and Q8C7D2). Immunogen sequence is well conserved in essentially all mammalian species.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRBN
      • AD-006
      • MRT2A
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50 kDa observed. 50.55/50.48 kDa (human isoform 1/2) and 50.88/50.81 kDa (mouse isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected cereblon in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications