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17-299 Anti-Cdc42 Immunoblotting Kit

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17-299
1 kit  Kit capacity: 10 immunoblots per kit
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      WB
      Description
      Catalogue Number17-299
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cdc42 Immunoblotting Kit
      OverviewAnti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog
      References
      Product Information
      Components
      • Anti-cdc42, (mouse monoclonal IgG1), Catalog # 05-542
      • Immunoblot Blocking Reagent (Cat.# 20-200)
      • Goat Anti-Mouse IgG, HRP conjugate (Cat.# 12-349)
      PresentationSee Certificate of Analysis for details
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cdc42 Immunoblotting Kit detects Cdc42 when performing western blots.
      Key Applications
      • Western Blotting
      Biological Information
      HostMouse
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
      Gene Symbol
      • CDC42
      • G25K
      • CDC42Hs
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
      SIZE: 191 amino acids; 21311 Da
      SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from HL-60 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment.
      Note: Catalog # 12-349, Goat Anti-Mouse IgG, HRP-conjugate is light sensitive, handle accordingly.
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 10 immunoblots per kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      17-299 04053252280443

      Documentation

      Anti-Cdc42 Immunoblotting Kit Certificates of Analysis

      TitleLot Number
      Anti-cdc42 Immunoblotting Kit 2912074
      Anti-cdc42 Immunoblotting Kit 2832143
      Anti-cdc42 Immunoblotting Kit 3105944
      Anti-cdc42 Immunoblotting Kit - 2459580 2459580
      Anti-cdc42 Immunoblotting Kit - 0701050665 0701050665
      Anti-cdc42 Immunoblotting Kit - 20130 20130
      Anti-cdc42 Immunoblotting Kit - 2043487 2043487
      Anti-cdc42 Immunoblotting Kit - 2064535 2064535
      Anti-cdc42 Immunoblotting Kit - 21680 21680
      Anti-cdc42 Immunoblotting Kit - 2212028 2212028

      References

      Reference overviewPub Med ID
      Impaired NK-cell migration in WAS/XLT patients: role of Cdc42/WASp pathway in the control of chemokine-induced beta2 integrin high-affinity state.
      Helena Stabile,Claudia Carlino,Cinzia Mazza,Silvia Giliani,Stefania Morrone,Lucia D Notarangelo,Luigi D Notarangelo,Angela Santoni,Angela Gismondi
      Blood  115  2010

      Show Abstract Full Text Article
      20130240 20130240
      Impaired natural and CD16-mediated NK cell cytotoxicity in patients with WAS and XLT: ability of IL-2 to correct NK cell functional defect.
      Angela Gismondi, Loredana Cifaldi, Cinzia Mazza, Silvia Giliani, Silvia Parolini, Stefania Morrone, Jordan Jacobelli, Elisabetta Bandiera, Luigi Notarangelo, Angela Santoni
      Blood  104  436-43  2004

      Show Abstract
      15001467 15001467

      Related Products & Applications

      Kit Component

      Catalog Number Description  
      12-349 Goat Anti-Mouse IgG Antibody, HRP conjugate Show Pricing & Availability
      20-200 Immunoblot Blocking Reagent Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents