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MAB4603 Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03

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MAB4603
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4603
      Replaces04-440; 04-1088
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
      Alternate Names
      • CPP32
      References
      Product Information
      FormatPurified
      PresentationLiquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1 μg/ml for 2 hours at RT.

      Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant full-length human caspase-3 protein
      Epitopelarge subunit & proform
      Clone3CSP03
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
      Gene Symbol
      • CASP3
      • apopain
      • CASP-3
      • procaspase3
      • CPP32B
      • SCA-1
      • CPP-32
      • Apopain
      • CPP32
      • Yama
      • EC 3.4.22.56 [Contains: Caspase-3 subunit p17
      • Caspase-3 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
      SIZE: 277 amino acids; 31608 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
      PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CASPASE-3 [CPP32] - 3526326 3526326
      MOUSE ANTI-CASPASE-3 [CPP32] MONOCLONAL ANTIBODY - 2386958 2386958

      References

      Reference overviewApplicationPub Med ID
      Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
      Suffert, G; Malterer, G; Hausser, J; Viiliäinen, J; Fender, A; Contrant, M; Ivacevic, T; Benes, V; Gros, F; Voinnet, O; Zavolan, M; Ojala, PM; Haas, JG; Pfeffer, S
      PLoS pathogens  7  e1002405  2011

      Show Abstract
      Western Blotting22174674 22174674
      Characterization of the interleukin-1beta-converting enzyme/ced-3-family protease, caspase-3/CPP32, in Hodgkin's disease: lack of caspase-3 expression in nodular lymphocyte predominance Hodgkin's disease.
      Izban, K F, et al.
      Am. J. Pathol., 154: 1439-47 (1999)  1999

      Show Abstract
      10329597 10329597
      Inverse correlation between apoptotic (Fas ligand, caspase-3) and angiogenic factors (VEGF, microvessel density) in squamous cell lung carcinomas.
      Volm, M, et al.
      Anticancer Res., 19: 1669-71 (1999)  1999

      Show Abstract
      10470099 10470099
      Expression of caspases 3, 6 and 8 is increased in parallel with apoptosis and histological aggressiveness of the breast lesion.
      Vakkala, M, et al.
      Br. J. Cancer, 81: 592-9 (1999)  1999

      Show Abstract
      10574243 10574243
      Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma.
      Virkajärvi, N, et al.
      Histopathology, 33: 432-9 (1998)  1998

      Show Abstract
      9839167 9839167
      In situ immunodetection of activated caspase-3 in apoptotic neurons in the developing nervous system.
      Srinivasan, A, et al.
      Cell Death Differ., 5: 1004-16 (1998)  1998

      Show Abstract
      9894607 9894607
      Colocalization of CPP-32 with apoptotic cells in human atherosclerotic plaques.
      Mallat, Z, et al.
      Circulation, 96: 424-8 (1997)  1997

      Show Abstract
      9244207 9244207
      Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
      Krajewska, M, et al.
      Cancer Res., 57: 1605-13 (1997)  1997

      Show Abstract
      9108467 9108467

      Brochure

      Title
      Comprehensive solutions for studying cell health - Life, death, and everything in between.

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies