AB3623 Anti-Caspase 3 Antibody, active (cleaved) form

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AB3623
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IF, IHC, IH(P), WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3623
      Replaces04-440; 04-1088
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 3 Antibody, active (cleaved) form
      Alternate Names
      • Apopain
      • Yama Protein
      • CASP3
      • SCA-1
      • procaspase3
      Background InformationCaspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.
      PresentationPurified rabbit polyclonal in buffer containing PBS, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 and has been published and validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), and Western Blot (WB).
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blot Analysis:
      1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
      Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.

      Immunohistochemistry:
      1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.

      Immunohistochemistry(paraffin):
      Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
      Biological Information
      ImmunogenCleavage specific synthetic peptide sequence (proprietary).
      Epitopeactive (cleaved) form
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
      Gene Symbol
      • CASP3
      • CPP-32
      • Yama
      • CASP-3
      • CPP32B
      • SCA-1
      • CPP32
      • EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
      SIZE: 277 amino acids; 31608 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
      PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Caspase 3 Antibody, active (cleaved) form SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase 3 Antibody, active (cleaved) form Certificates of Analysis

      TitleLot Number
      Anti-Caspase 3, active (cleaved) form3076465
      Anti-Caspase 3, active (cleaved) form3072358
      Anti-Caspase 3, active (cleaved) form3167005
      Anti-Caspase 3, active (cleaved) form3068447
      Anti-Caspase 3, active (cleaved) form - 21180032118003
      Anti-Caspase 3, active (cleaved) form - 23706542370654
      Anti-Caspase 3, active (cleaved) form - 23764232376423
      Anti-Caspase 3, active (cleaved) form - 23874642387464
      Anti-Caspase 3, active (cleaved) form - 24244812424481
      Anti-Caspase 3, active (cleaved) form - 24589572458957

      References

      Reference overviewApplicationSpeciesPub Med ID
      Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors.
      Gautier, HO; Evans, KA; Volbracht, K; James, R; Sitnikov, S; Lundgaard, I; James, F; Lao-Peregrin, C; Reynolds, R; Franklin, RJ; Káradóttir, RT
      Nature communications  6  8518  2015

      Show Abstract
      26439639 26439639
      Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model.
      Tarang, S; Doi, SM; Gurumurthy, CB; Harms, D; Quadros, R; Rocha-Sanchez, SM
      Frontiers in cellular neuroscience  9  52  2015

      Show Abstract
      25755634 25755634
      Folate deficiency decreases apoptosis of endometrium decidual cells in pregnant mice via the mitochondrial pathway.
      Liao, XG; Li, YL; Gao, RF; Geng, YQ; Chen, XM; Liu, XQ; Ding, YB; Mu, XY; Wang, YX; He, JL
      Nutrients  7  1916-32  2015

      Show Abstract
      25781218 25781218
      Dysregulation of astrocyte extracellular signaling in Costello syndrome.
      Krencik, R; Hokanson, KC; Narayan, AR; Dvornik, J; Rooney, GE; Rauen, KA; Weiss, LA; Rowitch, DH; Ullian, EM
      Science translational medicine  7  286ra66  2015

      Show Abstract
      Western Blotting25947161 25947161
      Impact of a deletion of the full-length and short isoform of p75NTR on cholinergic innervation and the population of postmitotic doublecortin positive cells in the dentate gyrus.
      Poser, R; Dokter, M; von Bohlen Und Halbach, V; Berger, SM; Busch, R; Baldus, M; Unsicker, K; von Bohlen Und Halbach, O
      Frontiers in neuroanatomy  9  63  2015

      Show Abstract
      26074780 26074780
      Facile bench-top fabrication of enclosed circular microchannels provides 3D confined structure for growth of prostate epithelial cells.
      Dolega, ME; Wagh, J; Gerbaud, S; Kermarrec, F; Alcaraz, JP; Martin, DK; Gidrol, X; Picollet-D'hahan, N
      PloS one  9  e99416  2014

      Show Abstract
      24945245 24945245
      Electroacupuncture-like stimulation at Baihui and Dazhui acupoints exerts neuroprotective effects through activation of the brain-derived neurotrophic factor-mediated MEK1/2/ERK1/2/p90RSK/bad signaling pathway in mild transient focal cerebral ischemia in rats.
      Cheng, CY; Lin, JG; Su, SY; Tang, NY; Kao, ST; Hsieh, CL
      BMC complementary and alternative medicine  14  92  2014

      Show Abstract
      Western Blotting24606810 24606810
      Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress.
      Suttkus, A; Rohn, S; Weigel, S; Glöckner, P; Arendt, T; Morawski, M
      Cell death & disease  5  e1119  2014

      Show Abstract
      Immunohistochemistry24625978 24625978
      The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis.
      Osz, K; Ross, M; Petrik, J
      Reproductive biology and endocrinology : RB&E  12  21  2014

      Show Abstract
      24628875 24628875
      Inhibition of proliferation and migration of luminal and claudin-low breast cancer cells by PDGFR inhibitors.
      Stalker, L; Pemberton, J; Moorehead, RA
      Cancer cell international  14  89  2014

      Show Abstract
      Immunofluorescence25253994 25253994

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      Life Science Research > Antibodies and Assays > Primary Antibodies