AB5889 Anti-Capsaicin Receptor Antibody, NT

View Products on Sigmaaldrich.com
AB5889
50 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, Pm WB, IHC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5889
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Capsaicin Receptor Antibody, NT
      Alternate Names
      • Vanilloid Receptor 1
      • VR1
      References
      Product Information
      FormatSerum
      PresentationLiquid. Contains 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunoblotting: 1:1,000

      Immunohistochemistry: 1:1,000

      Optimal working dilutions must be determined by the end user.

      APPLICATION NOTES FOR AB5889

      IMMUNOHISTOCHEMISTRY

      Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

      Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

      IMMUNOHISTOCHEMISTRY

      VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.

      WESTERN BLOT

      For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the N-terminus of rat VR1.
      EpitopeN-terminus
      HostRabbit
      SpecificityCapsaicin Receptor (VR1), N-terminal.

      SPECIES REACTIVITIES:

      Rat, mouse and primate. Other species have not been tested.
      Species Reactivity
      • Mouse
      • Rat
      • Primate
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCapsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
      Gene Symbol
      • TRPV1
      • VR1
      • OTRPC1
      • TrpV1
      • DKFZp434K0220
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8NER1 # Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
      SIZE: 839 amino acids; 94938 Da
      SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
      TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
      PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
      SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
      MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Capsaicin Receptor Antibody, NT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Capsaicin Receptor Antibody, NT Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY - 21277522127752
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY - 23705702370570
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY - 24350812435081
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL - 32265823226582
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL - 32755663275566
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL - 33665123366512
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL - 34506563450656
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY3022017
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY3029799
      RABBIT ANTI-CAPSAICIN RECEPTOR (VR1) N-TERMINAL POLYCLONAL ANTIBODY2905549

      References

      Reference overviewPub Med ID
      Cannabinoids attenuate the effects of aging upon neuroinflammation and neurogenesis.
      Marchalant, Yannick, et al.
      Neurobiol. Dis., 34: 300-7 (2009)  2009

      Show Abstract
      19385063 19385063
      TRPV1 stimulation triggers apoptotic cell death of rat cortical neurons.
      Hisashi Shirakawa,Tomoko Yamaoka,Kazuaki Sanpei,Hirotoshi Sasaoka,Takayuki Nakagawa,Shuji Kaneko
      Biochemical and biophysical research communications  377  2008

      Show Abstract
      18996081 18996081
      Immunocytochemical localization of the vanilloid receptor 1 (VR1): relationship to neuropeptides, the P2X3 purinoceptor and IB4 binding sites
      Guo, A. et al.
      Eur. J. Neurosci., 11(3):946-958 (1999)  1999

      10103088 10103088

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB5370 Anti-Capsaicin Receptor Antibody, CT Show Pricing & Availability
      AB5370P Anti-Capsaicin Receptor Antibody, CT Show Pricing & Availability
      AB5566 Anti-Capsaicin Receptor Antibody, CT Show Pricing & Availability
      AB9554 Anti-Capsaicin Receptor Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies