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MABF2181-100UL Anti-CXCR3 (CD183) Antibody, clone 173

MABF2181-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2181-100UL
      DescriptionAnti-CXCR3 (CD183) Antibody, clone 173
      Alternate Names
      • C-X-C chemokine receptor type 3
      • CXC-R3
      • Interferon-inducible protein 10 receptor
      • IP-10 receptor
      • CD183
      Background InformationC-X-C chemokine receptor type 3 (UniProt: O88410; also known as CXC-R3, CXCR-3, Interferon-inducible protein 10 receptor, IP-10 receptor, CD183) is encoded by the Cxcr3 (also known as Cmkar3) gene (Gene ID: 12766) in murine species.CXCR-3 is a multi-pass membrane protein of the G-protein coupled receptor family. It is highly expressed on effector Th1-type CD4+ and effector CD8+ cells and serves as a chemokine receptor for interferon-inducing ligands, CXCL9, CXCL10, and CXCL11. CXCR-3 is reported to play a critical role in the migration of T cells in the microenvironment of the peripheral tissue and lymphoid compartment, that facilitates the interaction of T cells with antigen presenting cells leading to the generation of effector and memory cells. CXCR-3 expression on effector T cells is shown to be essential for their entry into sites of inflammation. CXCR-3 is an N- glycosylated protein, which also undergoes sulfation on tyrosine 27 and 29. These sulfations are essential for its binding to CXCL10. Clone 173 can block receptor binding of CXCL10 and CXCL11, but not CXCL9 in murine cells. When injected into mice along with low dose of rapamycin, it is shown to prolong cardiac and islet allograft survival. (Ref.: Uppaluri, R., et al. (2008). Transplantation. 86(1): 137-147; Groom JR., and Luster, AD. (2011). Exp. Cell Res. 317(5); 620-631).
      References
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal antibody IgG in PBS without preservatives.
      Applications
      ApplicationAnti-CXCR3 (CD183), clone 173, Cat. No. MABF2181, is an Armenian hamster monoclonal antibody that detects C-X-C chemokine receptor type 3 and has been tested for use in Flow Cytometry, Inhibition Assay, and Radioimmunoassay.
      Key Applications
      • Flow Cytometry
      • Inhibition
      • Radioimmunoassay
      Application NotesRadioimmunoassay Analysis: A representative lot detected CXCR3 (CD183) in Radioimmunoassay applications (Uppaluri, R., et. al. (2008). Transplantation. 86(1):137-47).

      Inhibition Analysis: A representative lot inhibited CXCR3 binding of mCXCL10 and mCXCL11.(Uppaluri, R., et. al. (2008). Transplantation. 86(1):137-47).

      Flow Cytometry Analysis: A representative lot detected CXCR3 (CD183) in Flow Cytometry applications (Uppaluri, R., et. al. (2008). Transplantation. 86(1):137-47).
      Biological Information
      ImmunogenA multiple antigen peptide (MAPS8) corresponding to 37 amino acids from the N-terminal region of murine C-X-C chemokine receptor 3 (CXCR3).
      EpitopeN-terminus
      Clone173
      ConcentrationPlease refer to lot specific datasheet.
      HostArmenian Hamster
      SpecificityClone 173 is an Armenian Hamster monoclonal antibody that specifically detects CXCR3 on cells from wild-type, but not from CXCR3-/- mice.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cxcr3
      • Cmkar3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight41.02 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

      Flow Cytometry Analysis: 1 µg of this antibody detected CXCR3 (CD183) in one million mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CXCR3 (CD183) Antibody, clone 173 Certificates of Analysis

      TitleLot Number
      Anti-CXCR3 (CD183), clone 173 - Q3238879Q3238879

      References

      Reference overviewPub Med ID
      Prolongation of cardiac and islet allograft survival by a blocking hamster anti-mouse CXCR3 monoclonal antibody.
      Uppaluri, R; Sheehan, KC; Wang, L; Bui, JD; Brotman, JJ; Lu, B; Gerard, C; Hancock, WW; Schreiber, RD
      Transplantation  86  137-47  2008

      Show Abstract
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