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ABN1646 Anti-CDC42 Antibody, Brain Isoform-specific

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ABN1646
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1646
      DescriptionAnti-CDC42 Antibody, Brain Isoform-specific
      Alternate Names
      • Cell division control protein 42 homolog
      • G25K GTP-binding protein
      Background InformationCell division control protein 42 homolog (UniProt: P60953; also known as G25K GTP-binding protein) is encoded by the CDC42 gene (Gene ID 998) in human. CDC42 homolog is a plasma membrane-associated small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state. Its activity is regulated by guanine nucleotide exchange factors (GEFs), which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) that increase the GTP hydrolysis activity, and GDP dissociation inhibitors, which inhibit the dissociation of the nucleotide from the GTPase. In the active state it binds to a variety of effector proteins to regulate cellular responses. Cdc42 is known to regulate the dynamic organization of the cytoskeleton and membrane trafficking for physiologic processes such as cell proliferation, motility, polarity, cytokinesis and growth. Deregulation of Cdc42 is found in several pathogenic processes such as cancer, cardiovascular diseases, and neuronal degenerative diseases. It is involved in epithelial cell polarization processes and regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. During the prometaphase stage, it localizes to spindle and moves to the central spindle as cells progress through anaphase to telophase.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CDC42, Brain Isoform-specific, Cat. No. ABN1646, is a highly specific rabbit polyclonal antibody that targets CDC42 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected GFP-tagged recombinant CDC42 brain isoform (Cdc42-palm), but not the placenta isoform Cdc42-prenyl exogenously expressed in COS-7 cells (Courtesy provided by Rujun).

      Immunocytochemistry Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) at the axon tip by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized DIV3cortical neurons from Zdhhc8-deficient mice (Mukai, J., et al. (2015). Neuron. 86(3):680-695).

      Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in whole cell lysate and among purified palmitoylated proteins (PPs) from cultured rat cortical embryonic neurons (Kang, R., et al. (2008). Nature. 456(7224):904-909).

      Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in rat brain membrane fractions (Kang, R., et al. (2008). Nature. 456(7224):904-909).
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal end sequence of mature human CDC42 brain isoform.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody is purified from an antiserum that has been shown to specifically detect recombinant human CDC42 brain isoform Cdc42-palm (UniProt P60953-1) with or without C188S mutation, but not the placenta isoform Cdc42-prenyl (UniProt P60953-2) by Western blotting analysis (Kang, R., et al. (2008). Nature. 456(7224):904-909). Note that the designation of CDC42 isoform 1 and 2 by Kang et al. (2008) is the exact opposite of that by UniProt P60953 (human) and P60766 (mouse), while both Kang et al. (2008) and UniProt Q8CFN2 (rat) designate the rat CDC42 brain isoform as isoform 2.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDC42
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~21 kDa observed. 20.95/20.95/20.92 kDa (human/mouse/rat mature CDC42 brain isoform; propeptide a.a. 189-191 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse hippocampus tissue lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected CDC42 brain isoform (Cdc42-palm) in 10 µg of mouse hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications