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MAB1379 Anti-CD54 Antibody, extracellular, clone 84H10

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MAB1379
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, IHC, FUNCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1379
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD54 Antibody, extracellular, clone 84H10
      Alternate Names
      • ICAM-1
      Background InformationThis molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells.
      References
      Product Information
      FormatPurified
      PresentationAffinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesFlow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells.

      Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.

      Immunoprecipitation: 5 μg/ 10e7 cells

      Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenK562 cell line
      Epitopeextracellular
      Clone84H10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryICAM1 (CD54) is typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. ICAM1 is also exploited by Rhinovirus as a receptor.
      Gene Symbol
      • ICAM1
      • P3.58
      • BB2
      • CD54
      • ICAM-1
      Non-Reactive Species
      • Canine
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05362 # ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). In case of rhinovirus infection acts as a cellular receptor for the virus. Also acts as receptor for some coxsackieviruses.
      SIZE: 532 amino acids; 57825 Da
      SUBUNIT: Interacts with human herpesvirus 8 MIR2 protein (Probable).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
      SIMILARITY: SwissProt: P05362 ## Belongs to the immunoglobulin superfamily. ICAM family. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20ºC. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD54 Antibody, extracellular, clone 84H10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD54 Antibody, extracellular, clone 84H10 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2122406 2122406
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2146409 2146409
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2360850 2360850
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2376422 2376422
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2420365 2420365
      MOUSE ANTI HUMAN CD54 MONOCLONAL ANTIBODY - 2436343 2436343
      MOUSE ANTI HUMAN CD54 - 3494020 3494020
      MOUSE ANTI HUMAN CD54 - 3612259 3612259
      MOUSE ANTI HUMAN CD54 - 3733766 3733766
      MOUSE ANTI HUMAN CD54 -2545364 2545364

      References

      Reference overviewPub Med ID
      Cellular sources of MMP-7, MMP-13 and MMP-28 in ulcerative colitis.
      Timo Rath,Martin Roderfeld,Jörg Michael Halwe,Annette Tschuschner,Elke Roeb,Jürgen Graf
      Scandinavian journal of gastroenterology  45  2010

      Show Abstract
      20568971 20568971
      Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin.
      Burns, MP; DePaola, N
      American journal of physiology. Heart and circulatory physiology  288  H194-204  2005

      Show Abstract
      15331359 15331359
      Synergistic induction of intercellular adhesion molecule-1 by the human cytomegalovirus transactivators IE2p86 and pp71 is mediated via an Sp1-binding site.
      Martina Kronschnabl, Thomas Stamminger
      The Journal of general virology  84  61-73  2003

      Show Abstract
      12533701 12533701

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies