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MAB4211 Anti-CD34 Class I Antibody, clone B1-3C5

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100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IFMPurifiedMonoclonal Antibody
      Catalogue NumberMAB4211
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD34 Class I Antibody, clone B1-3C5
      Product Information
      PresentationThe antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
      Quality LevelMQ100
      ApplicationDetect CD34 Class I using this Anti-CD34 Antibody (Class 1), clone B1-3C5 validated for use in FC & IF.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      Application NotesIdentification of CD34 positive cells is important for the diagnosis and classification of acute myeloid leukemia (AML). It is especially useful in patients with poorly differentiated myeloblasts, as well as in myeloblastic crises of chronic granulocytic leukemia. It has been recommended that this antibody be used as part of a panel for screening of leukemia (Batinic et al., 1989).


      1. Flow cytometry and indirect immunofluorescence 1:25

      Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl of buffer.

      2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.
      Biological Information
      ImmunogenKG1 human acute myelogenous cell line.
      SpecificityThis antibody belongs to CD34 (assigned by the Third International Workshop on Leukocyte Differentiation Antigens, Oxford, 1987; Hogg & Horton, 1987). It reacts with a 120kD glycoprotein found on progenitor cell populations in human bone marrow and foetal liver (Katz et al., 1985). This antigen is normally restricted to immature human haemopoietic precursor cells (Peschel & Koller, 1989).

      Cell reactivity (Tindle et al., 1985):

      Normal: No reaction with normal peripheral lymphocytes, granulocytes, monocytes, erythrocytes and platelets. Reacts with <4% of cells in normal bone marrow.

      Clinical: Reaction

      Acute myeloid leukemia (AML, M1 and M2) positive

      Null lymphoblastic leukemia (Null ALL) positive

      Common acute lymphoblastic leukemia (CALL) positive

      Myeloblastic and lymphoblastic crises of chronic

      granulocytic leukemia (CGL) positive

      Chronic myeloid leukemia (CML) negative

      T cell acute lymphoblastic leukemia (TALL) negative

      B cell acute lymphoblastic leukemia (BALL) negative

      Chronic lymphocytic leukemia (CLL) negative

      Cell lines:

      Reacts with early myeloblast line KG1. No reaction with null, B or T lymphoid, erythroid or later myeloid cell lines.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
      Gene Symbol
      • CD34
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
      SIZE: 385 amino acids; 40716 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
      DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
      PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
      DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
      SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in the United States.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. Product stable for 6 months when stored as directed.

      WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-CD34 Class I Antibody, clone B1-3C5 SDS


      Safety Data Sheet (SDS) 

      Anti-CD34 Class I Antibody, clone B1-3C5 Certificates of Analysis

      TitleLot Number
      Mouse monoclonal antibody : Human CD34 , purified - ME8NN9348 ME8NN9348
      Mouse monoclonal antibody : Human CD34 , purified - ME8PN2819 ME8PN2819
      Mouse monoclonal antibody : Human CD34 , purified - ME9EN5645 ME9EN5645
      Mouse monoclonal antibody : Human CD34 , purified - ME9JN2337 ME9JN2337
      Mouse monoclonal antibody: Human CD34 , purified - ME8NN3241 ME8NN3241


      Reference overviewPub Med ID
      Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation.
      Park, IS; Chung, PS; Ahn, JC
      PloS one  10  e0122776  2015

      Show Abstract
      26065900 26065900
      A phase I clinical trial of the treatment of Crohn's fistula by adipose mesenchymal stem cell transplantation.
      García-Olmo, Damián, et al.
      Dis. Colon Rectum, 48: 1416-23 (2005)  2005

      15933795 15933795

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      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies