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09-143 Anti-Brk Antibody

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09-143
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-143
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Brk Antibody
      Alternate Names
      • PTK6
      • protein tyrosine kinase 6
      • breast tumor kinase
      • protein-tyrosine kinase BRK
      References
      Product Information
      FormatAffinity Purified
      Presentation50 µg of affinity purified rabbit polyclonal IgG in 0.02 M PBS, 250 mM sodium chloride containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Brk using this Anti-Brk Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH conjugated synthetic peptide corresponding to the N-terminal region of human Brk.
      HostRabbit
      SpecificityRecognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
      Gene Symbol
      • PTK6
      • BRK
      • FLJ42088
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
      SIZE: 451 amino acids; 51834 Da
      SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
      TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
      SIMILARITY: SwissProt: Q13882 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight52 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore for 1 year at 2-8°C from date of shipment.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      09-143 04053252736766

      Documentation

      Anti-Brk Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brk Antibody Certificates of Analysis

      TitleLot Number
      Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) - 2399454 2399454
      Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) 2869706
      Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) 2868378
      Anti-Brk (Affinity Purified Rabbit Polyclonal IgG) - 3378622 3378622
      Anti-Brk - 2530946 2530946
      Anti-Brk - 3179970 3179970
      Anti-Brk - DAM1576945 DAM1576945
      Anti-Brk - DAM1597375 DAM1597375
      Anti-Brk - DAM1634449 DAM1634449
      Anti-Brk - DAM1644571 DAM1644571

      References

      Reference overviewPub Med ID
      Growth response of Escherichia coli ATCC 35218 adapted to several concentrations of sodium benzoate and potassium sorbate.
      Santiesteban-López NA, Rosales M, Palou E, López-Malo A
      J Food Prot  72  2301-7.  2009

      Show Abstract
      19903392 19903392
      Expression and oncogenic role of Brk (PTK6/Sik) protein tyrosine kinase in lymphocytes
      Kasprzycka, Monika, et al
      Am J Pathol, 168:1631-41 (2006)  2006

      16651629 16651629
      The intracellular tyrosine kinase Brk sensitizes non-transformed cells to inducers of apoptosis
      Haegebarth, Andrea, et al
      Cell Cycle, 4:1239-46 (2005)  2005

      16082217 16082217
      Interaction between Brk kinase and insulin receptor substrate-4
      Qiu, Haoqun, et al
      Oncogene, 24:5656-64 (2005)  2005

      15870689 15870689
      Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition
      Qiu, Haoqun and Miller, W Todd
      J Biol Chem, 277:34634-41 (2002)  2002

      12121988 12121988

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies