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MABC1190 Anti-Bin1 Antibody, clone 2F11

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MABC1190
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1190
      DescriptionAnti-Bin1 Antibody, clone 2F11
      Alternate Names
      • Myc box-dependent-interacting protein 1
      • Amphiphysin II
      • Amphiphysin-like protein
      • Box-dependent myc-interacting protein 1
      • Bridging integrator 1
      Background InformationMyc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. It contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190, is a mouse monoclonal antibody that detects Bridging integrator 1 (Bin1) protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.

      Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).

      Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the BAR domain (aa 29-276) from the N-terminal half of human Myc box-dependent-interacting protein 1 (Bin1).
      Epitopedomain of:
      Clone2F11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2F11 recognizes an epitope within the N-terminal BAR domain of Bin 1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Chicken
      • Mouse
      Species Reactivity NoteChicken, Human, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BIN1
      • AMPHL
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight64.70 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human cerebellum and human skeletal muscle tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Bin1 in human cerebellum and human skeletal muscle tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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