Product Information
Applications
Application ReferencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
Key Applications Immunoprecipitation
Paraffin Sections
Flow Cytometry
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesFlow Cytometry (see application references)
Immunoblotting (2 µg/ml, see application references)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (1 µg/ml)
Application CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
ImmunogenHuman
Clone4D7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Bcl-2 (Ab-3) Mouse mAb (4D7) SDS

Title

Safety Data Sheet (SDS) 

Anti-Bcl-2 (Ab-3) Mouse mAb (4D7) Certificates of Analysis

TitleLot Number
OP91

References

Reference overview
Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

Citations

Title
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision03-October-2007 RFH
    ApplicationFlow Cytometry (see application references)
    Immunoblotting (2 µg/ml, see application references)
    Immunofluorescence (see application references)
    Immunoprecipitation (see application references)
    Paraffin Sections (1 µg/ml)
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse SP2/0 cells. Recognizes the ~24 kDa Bcl-2 protein.
    BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
    HostMouse
    Immunogen speciesHuman
    Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
    Clone4D7
    IsotypeIgG₁
    Specieshuman, not mouse, not rat
    Positive controlMCF-7 cells or breast carcinoma or lymph node tissue
    Negative controlBrain tissue
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesLarsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
    Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
    Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
    Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
    Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
    Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
    Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
    Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
    Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
    Citation
  • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
  • Application referencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.

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    Life Science Research > Antibodies and Assays > Primary Antibodies