GF35L Anti-BDNF Mouse mAb (35928.11)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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GF35L-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
      Catalogue NumberGF35L
      Brand Family Calbiochem®
      SynonymsAnti-Brain Derived Neurotrophic Factor
      References
      ReferencesRiccio, A., et al. 1997. Science 277 1097.
      Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
      Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
      Gotz, R., et al. 1994. Nature 372, 266.
      Snider, W.D. 1994. Cell 77, 627.
      Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, 5% trehalose.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Neutralization Studies
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (0.5-1 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Immunohistochemistry (5-15 µg/ml)
      Neutralization Studies (see comments)
      Application CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
      ImmunogenHuman
      Clone35928.11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-BDNF Mouse mAb (35928.11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BDNF Mouse mAb (35928.11) Certificates of Analysis

      TitleLot Number
      GF35L

      References

      Reference overview
      Riccio, A., et al. 1997. Science 277 1097.
      Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
      Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
      Gotz, R., et al. 1994. Nature 372, 266.
      Snider, W.D. 1994. Cell 77, 627.
      Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-April-2010 JSW
      SynonymsAnti-Brain Derived Neurotrophic Factor
      ApplicationELISA (0.5-1 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Immunohistochemistry (5-15 µg/ml)
      Neutralization Studies (see comments)
      DescriptionImmunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
      BackgroundNeurotrophic factors define a family of related proteins, which are important in the development, maintenance, and regulation of neurons in both the peripheral and central nervous system. In addition to the prototypical nerve growth factor (NGF), several other factors have been identified which interact with a family of tyrosine kinase receptors. TrkA is the kinase signaling receptor for NGF, while TrkB mediates signaling for both brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). TrkC is the primary receptor for neurotrophin-3 (NT-3), although this neurotrophin can also activate TrkA and TrkB receptors under certain conditions. Axotomy-induced cell death is one experimental paradigm that has been used to discriminate between the effects of various neurotrophic factors on the survival of different neuronal populations. BDNF, NT-3, and NT-4/5 act as motor neuron survival factors, whereas NGF has no effect on the survival of spinal cord motor neurons. NGF is neuroprotective for cholinergic neurons in the central nervous system. Retrogradely transported NGF-TrkA has also been shown to signal the CREB transcription factor in sympathetic neurons. A putative new member of this gene family, neurotrophin-6 (NT-6), has also been identified from the fish Xephophorus. Like NGF, NT-6 promotes the survival of sympathetic and sensory neurons, albeit with a lower potency.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
      Clone35928.11
      IsotypeIgG₁
      Specieshuman
      FormLyophilized
      FormulationLyophilized from PBS, 5% trehalose.
      PreservativeNone
      CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesRiccio, A., et al. 1997. Science 277 1097.
      Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
      Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
      Gotz, R., et al. 1994. Nature 372, 266.
      Snider, W.D. 1994. Cell 77, 627.
      Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.