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MABF2198-100UL Anti-BCA-225 (BRST-1) Antibody, clone CU-18

MABF2198-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2198-100UL
      DescriptionAnti-BCA-225 (BRST-1) Antibody, clone CU-18
      Alternate Names
      • Breast Carcinoma Associated Glycoprotein
      • Breast cancer antigen 225
      • BRST-1
      Background InformationBCA-225 is a glycoprotein present in human breast carcinoma cells that has been reported to show a restricted distribution in other human tissues. It is secreted by the T47D breast carcinoma cell line and its expression is common in adenocarcinomas of the breast (~98%), ovary (~ 80%), and lung (~74%) but is reported to be rare in adenocarcinomas of the gastrointestinal tract (~ 10%). Clone CU18 specifically recognizes BCA-225 in breast carcinoma an does not react with other benign or malignant tissues. BCA-225 is considered to be a a highly sensitive marker of mammary tissue and intracytoplasmic staining has been shown in over 95% of breast carcinomas, both in the primary tumor and in its metastases. (Ref.: Han, J-H., et al. (2003). Arch. Pathol. Lab. Med. 127:1330 1334; Mesa-Tejada, R., et al. (1988). Am. J. Pathol. 130(2); 305-314).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BCA-225 (BRST-1), clone CU-18, Cat. No. MABF2198, is a mouse monoclonal antibody that detects BCA-225 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected BCA-225 (BRST-1) in ELISA applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).

      Western Blotting Analysis: A representative lot detected BCA-225 (BRST-1) in Western Blotting applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).

      Immunocytochemistry Analysis: A representative lot detected BCA-225 (BRST-1) in Immunocytochemistry applications (Mesa-Tejada, R., et. al. (1988). Am J Pathol. 130(2):305-14).
      Biological Information
      ImmunogenBCA-225 protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
      CloneCU18
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CU18 is a mouse monoclonal antibody that detects human breast carcinoma associated glycoprotein BCA-225.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight225-250 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected BCA-225 (BRST-1) in human breast cancer tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-BCA-225 (BRST-1) Antibody, clone CU-18 Certificates of Analysis

      TitleLot Number
      Anti-BCA-225 (BRST-1), clone CU-18 - Q3226514Q3226514

      References

      Reference overviewPub Med ID
      Immunocytochemical distribution of a breast carcinoma associated glycoprotein identified by monoclonal antibodies.
      Mesa-Tejada, R; Palakodety, RB; Leon, JA; Khatcherian, AO; Greaton, CJ
      Am J Pathol  130  305-14  1988

      Show Abstract
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