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MAB3504-I Anti-Apaf-1 Antibody, clone 13F11

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWBRPurifiedMonoclonal Antibody
      Catalogue NumberMAB3504-I
      DescriptionAnti-Apaf-1 Antibody, clone 13F11
      Alternate Names
      • APAF
      • APAF-1
      • APAF1
      • apoptotic peptidase activating factor 1
      • apoptotic protease activating factor 1
      • Apoptotic protease-activating factor 1
      • CED4
      • DKFZp781B1145
      • KIAA0413
      • Apoptotic protease-activating factor 1
      Background InformationApoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.
      Product Information
      PresentationPurified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Apaf-1, clone 13F11, is a rat monoclonal antibody that detects Apaf-1 in mouse and rat. It has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 ug/mL from a representative lot detected Apaf-1 in mouse bone marrow tissue lysate.

      Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Vaughn, A.E., et. al. (2007). Cell Death Differ. 14(5):973-81; Wright, K.M., et. al. (2004). J Cell Biol. 167(2):303-13).
      Biological Information
      ImmunogenA recombinant fragment corresponding to the first 97 amino acids of mouse Apaf-1.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 13F11 specifically detects Apaf-1 in mouse and rat. It targets an epitope within the first 97 amino acids in the N-terminal region.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Apaf1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~141 kDa observed; 141.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse lymph node tissue lysate.

      Western Blotting Analysis: 1 ug/mL of this antibody detected Apaf-1 in mouse lymph node tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information