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07-383 Anti-Akt3/PKBγ Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-383
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Akt3/PKBγ Antibody
      Product Information
      FormatAffinity Purified
      • IGF-I stimulated HEK293 cell lysates
      PresentationPurified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Quality LevelMQ100
      ApplicationAnti-Akt3/PKBγ Antibody is an antibody against Akt3/PKBγ for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 119-131 of human Akt3/PKBgamma
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Akt3/PKBγ.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
      Gene Symbol
      • AKT3
      • PRKBG
      • DKFZP434N0250
      • PKBG
      • RAC-gamma
      • RAC-PK-gamma
      • STK-2
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y243 # IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
      SIZE: 479 amino acids; 55775 Da
      SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.
      TISSUE SPECIFICITY: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
      DOMAIN:SwissProt: Q9Y243 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight~60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitating active Akt3/PKBγ from IGF-I stimulated HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Akt3/PKBγ Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Akt3/PKBγ Antibody Certificates of Analysis

      TitleLot Number
      Anti-Akt3/PKB - 20731362073136
      Anti-Akt3/PKB - 22104052210405
      Anti-Akt3/PKB - JBC1813178JBC1813178
      Anti-Akt3/PKB - JBC1913127JBC1913127
      Anti-Akt3/PKB - JBC1936145JBC1936145
      Anti-Akt3/PKBg - DAM1586912DAM1586912
      Anti-Akt3/PKBgamma; - 2424324243
      Anti-Akt3/PKBgamma; - 2650826508
      Anti-Akt3/PKBγ - 31836413183641
      Anti-Akt3/PKBγ -25575832557583


      Reference overviewApplicationPub Med ID
      Cellular context-mediated Akt dynamics regulates MAP kinase signaling thresholds during angiogenesis.
      Hellesøy, M; Lorens, JB
      Molecular biology of the cell  26  2698-711  2015

      Show Abstract
      26023089 26023089
      Inhibition of HSP27 alone or in combination with pAKT inhibition as therapeutic approaches to target SPARC-induced glioma cell survival.
      Schultz, CR; Golembieski, WA; King, DA; Brown, SL; Brodie, C; Rempel, SA
      Molecular cancer  11  20  2012

      Show Abstract
      22480225 22480225
      Nonredundant functions for Akt isoforms in astrocyte growth and gliomagenesis in an orthotopic transplantation model.
      Endersby, R; Zhu, X; Hay, N; Ellison, DW; Baker, SJ
      Cancer research  71  4106-16  2011

      Show Abstract
      21507933 21507933
      The Akt isoforms are present at distinct subcellular locations.
      Santi, SA; Lee, H
      American journal of physiology. Cell physiology  298  C580-91  2010

      Show Abstract
      20018949 20018949
      Use of RNA interference-mediated gene silencing and adenoviral overexpression to elucidate the roles of AKT/protein kinase B isoforms in insulin actions
      Katome, T., et al
      J Biol Chem, 278:28312-23 (2003)  2003

      Immunoblotting (Western)12734182 12734182
      Characterization of PDK2 activity against protein kinase B gamma.
      Hodgkinson, Conrad P, et al.
      Biochemistry, 41: 10351-9 (2002)  2002

      Show Abstract
      12162751 12162751
      Akt/protein kinase B isoforms are differentially regulated by epidermal growth factor stimulation
      Okano, J., et al
      J Biol Chem, 275:30934-42 (2000)  2000

      Immunoblotting (Western)10908564 10908564
      Sources of supplementary materials for health instruction.
      American journal of public health and the nation's health  39  1949

      Full Text Article
      15392601 15392601

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      Life Science Research > Antibodies and Assays > Primary Antibodies