04-796 Anti-Akt1/PKBα Antibody, clone AW24 | 04-796

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IP Rb Purified Monoclonal Antibody
      Catalogue Number04-796
      DescriptionAnti-Akt1/PKBα Antibody, clone AW24 | 04-796
      Alternate Names
      • AKT1 kinase
      • Protein kinase B
      • RAC-alpha serine/threonine-protein kinase
      • rac protein kinase alpha
      • v-akt murine thymoma viral oncogene homolog 1
      Background InformationThe serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Product Information
      • HEK293 cell lysate.
      PresentationCultured supernantant containing 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Akt1 Antibody, clone AW24 detects level of Akt1/PKBα & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Biological Information
      ImmunogenSythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.
      ConcentrationCultured supernantant containing 0.05% sodium azide.
      SpecificityThis antibody recognizes Akt1/PKBα.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryInhibits JUN kinase activation and mediates inhibition of apoptosis.
      Gene Symbol
      • AKT
      • C-AKT
      • EC
      • MGC99656
      • PKB
      • PKB-ALPHA
      • PRKBA
      • RAC
      • RAC-ALPHA
      • RAC-PK-alpha
      UniProt Number
      UniProt SummaryFUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
      SUBUNIT STRUCTURE: Interacts with AGAP2 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.

      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane.The AGC-kinase C-terminal mediates interaction with THEM4.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
      INVOLVEMENT IN DISEASE:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.
      Defects in AKT1 are associated with colorectal cancer (CRC).Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.
      Molecular Weight~60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from human HEK293 cells.
      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Akt1/PKBα in RIPA lysates from human HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information


      Anti-Akt1/PKBα Antibody, clone AW24 | 04-796 SDS


      Safety Data Sheet (SDS) 

      Anti-Akt1/PKBα Antibody, clone AW24 | 04-796 Certificates of Analysis

      TitleLot Number
      Anti-Akt1/PKB#945;, clone AW24 - NG1811033NG1811033
      Anti-Akt1/PKB, clone AW24 - 20458722045872
      Anti-Akt1/PKB, clone AW24 - 23299502329950
      Anti-Akt1/PKB, clone AW24 - NG1734227NG1734227
      Anti-Akt1/PKB, clone AW24 - NG1843408NG1843408
      Anti-Akt1/PKB, clone AW24 - NRG1674335NRG1674335
      Anti-Akt1/PKBα, clone AW24 - 34072743407274
      Anti-Akt1/PKBα, clone AW24 -27162942716294
      Anti-Akt1/PKBα, clone AW24 -27690162769016


      Reference overviewPub Med ID
      Transformation by Tribbles homolog 2 (Trib2) requires both the Trib2 kinase domain and COP1 binding.
      Keeshan, K; Bailis, W; Dedhia, PH; Vega, ME; Shestova, O; Xu, L; Toscano, K; Uljon, SN; Blacklow, SC; Pear, WS
      Blood  116  4948-57  2010

      Show Abstract Full Text Article
      20805362 20805362

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