Product Information
Applications
Application ReferencesImmunoblotting Stiewe T., et al. 2000. Cancer Res. 60, 3957. Nishihara, A., et al. 1999. J. Biol. Chem. 274, 28716. Sanchez-Prieto, R., et al. 1999. Nat. Med. 5, 1076. van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunocytochemistry van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunofluoresecence Harlow, E., et al. 1986. Mol. Cell. Biol. 6, 1579. Harlow, E., et al. 1985. J. Virol. 3, 533. Original Clone Harlow, E., et al. 1985. J. Virol. 3, 533. Paraffin Sections Nemunaitis, H., et al. 2000 Cancer Res. 60, 6359.
Key Applications Immunofluorescence
Immunoprecipitation
Paraffin Sections
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunofluorescence (2-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg antibody/mg total protein)
Immunoblotting (1 µg/ml; see application references)
Immunocytochemistry (see application references)
Frozen Sections (2-4 µg/ml)
Paraffin Sections (2-4 µg/ml; heat pre-treatment required; see application references)
Application CommentsImmunoprecipitates the adenovirus 2 and 5 E1A proteins (3 bands at ~30-50 kDa) as well as associated proteins. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenadenovirus 2 E1A protein
CloneM73
HostMouse
IsotypeIgG2a
Species Reactivity
  • Adenovirus Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Adenovirus 2 E1A Mouse mAb (M73) SDS

Title

Safety Data Sheet (SDS) 

Anti-Adenovirus 2 E1A Mouse mAb (M73) Certificates of Analysis

TitleLot Number
DP11

References

Reference overview
Whyte, P., et al. 1998. Nature 334, 124.
Ziff, E. et al. 1985. Cell 40, 705.
Houweling, A., et al. 1980. Virology 105, 537.
Berk, A.J., et al. 1979. Cell 17, 935.
Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
Shiroki, K., et al. 1979. Virology 95, 127.
Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
Graham, F.L., et al. 1974. Cold Spring Harbor Symp. Quant. Biol. 39, 637.

Citations

Title
  • Ricardo Sanchez-Prieto, et al. (1999) An Association Between Viral Genes and Human Oncogenic Alterations: The Adenovirus E1A Induces the Ewing Tumor Fusion Transcript EWS-FLI1. Nature Medicine 5, 1076-1079.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision30-January-2009 JSW
    ApplicationImmunofluorescence (2-5 µg/ml, see application references)
    Immunoprecipitation (1-2 µg antibody/mg total protein)
    Immunoblotting (1 µg/ml; see application references)
    Immunocytochemistry (see application references)
    Frozen Sections (2-4 µg/ml)
    Paraffin Sections (2-4 µg/ml; heat pre-treatment required; see application references)
    DescriptionProtein A purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes adenovirus-infected cells and tissue.
    BackgroundThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the leftmost 11% of the viral genome and consists of two transcriptional units, E1A and E1B. Region E1A is sufficient for partial transformation and immortalization of primary cells, whereas the E1B function is normally required for complete transformation. In addition to their essential role in transformation, E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection, and are able to either activate or repress the transcription of specific cellular genes. E1A oncogene proteins form specific complexes with cellular proteins including the anti-oncogene retinoblastoma susceptibility gene product, p105. The consequence of this interaction is inhibition of the cell cycle arresting function of p105.
    HostMouse
    Immunogenadenovirus 2 E1A protein
    CloneM73
    IsotypeIgG2a
    Speciesadenovirus infected cells
    Positive controlHEK293 cells or adenovirus-infected tissue
    Negative controlHS27 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsImmunoprecipitates the adenovirus 2 and 5 E1A proteins (3 bands at ~30-50 kDa) as well as associated proteins. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
    Toxicity Standard Handling
    ReferencesWhyte, P., et al. 1998. Nature 334, 124.
    Ziff, E. et al. 1985. Cell 40, 705.
    Houweling, A., et al. 1980. Virology 105, 537.
    Berk, A.J., et al. 1979. Cell 17, 935.
    Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
    Shiroki, K., et al. 1979. Virology 95, 127.
    Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
    Graham, F.L., et al. 1974. Cold Spring Harbor Symp. Quant. Biol. 39, 637.
    Citation
  • Ricardo Sanchez-Prieto, et al. (1999) An Association Between Viral Genes and Human Oncogenic Alterations: The Adenovirus E1A Induces the Ewing Tumor Fusion Transcript EWS-FLI1. Nature Medicine 5, 1076-1079.
  • Application referencesImmunoblotting Stiewe T., et al. 2000. Cancer Res. 60, 3957. Nishihara, A., et al. 1999. J. Biol. Chem. 274, 28716. Sanchez-Prieto, R., et al. 1999. Nat. Med. 5, 1076. van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunocytochemistry van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunofluoresecence Harlow, E., et al. 1986. Mol. Cell. Biol. 6, 1579. Harlow, E., et al. 1985. J. Virol. 3, 533. Original Clone Harlow, E., et al. 1985. J. Virol. 3, 533. Paraffin Sections Nemunaitis, H., et al. 2000 Cancer Res. 60, 6359.

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    Categories

    Life Science Research > Antibodies and Assays > Primary Antibodies