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07-1286 Anti-ATM Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-1286
      Trade Name
      • Upstate
      DescriptionAnti-ATM Antibody
      Alternate Names
      • A-T, mutated
      • AT mutated
      • ataxia telangiectasia mutated
      • ataxia telangiectasia mutated (includes complementation groups A, C and D)
      • ataxia telangiectasia mutated protein
      • ATM
      • human phosphatidylinositol 3-kinase homolog
      • serine-protein kinase ATM
      • TEL1
      • telomere maintenance 1, homolog
      Background InformationATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
      Product Information
      FormatAffinity Purified
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-ATM Antibody detects level of ATM & has been published & validated for use in WB & IF.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected ATM in 10 µg of HeLa nuclear extract.
      Immunocytochemistry Analysis: A representative lot detected ATM in HeLa and NIH/3T3 cells.
      Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected ATM in NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to sequence derived from the Abl-interacting domain of human ATM.
      EpitopeAbl-interacting domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityDetects ATM.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Rat, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1.
      This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • AT1
      • ATA
      • ATC
      • ATD
      • ATDC
      • ATE
      • DKFZp781A0353 2
      • EC 3
      • MGC74674
      • TEL1
      • TELO1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight>260 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ATM in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information


      Anti-ATM Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-ATM Antibody Certificates of Analysis

      TitleLot Number
      Anti-ATM - 2383528 2383528
      Anti-ATM - 2049807 2049807
      Anti-ATM - 2216870 2216870
      Anti-ATM - 2279524 2279524
      Anti-ATM - 3022872 3022872
      Anti-ATM - 3277587 3277587
      Anti-ATM - 3511609 3511609
      Anti-ATM - 3723474 3723474
      Anti-ATM - 3891018 3891018
      Anti-ATM - JBC1771122 JBC1771122


      Reference overviewApplicationPub Med ID
      Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment.
      Ip, LR; Poulogiannis, G; Viciano, FC; Sasaki, J; Kofuji, S; Spanswick, VJ; Hochhauser, D; Hartley, JA; Sasaki, T; Gewinner, CA
      Oncotarget  6  10548-62  2015

      Show Abstract
      Western Blotting25868852 25868852
      Characterization of LGALS3 (galectin-3) as a player in DNA damage response.
      Carvalho, RS; Fernandes, VC; Nepomuceno, TC; Rodrigues, DC; Woods, NT; Suarez-Kurtz, G; Chammas, R; Monteiro, AN; Carvalho, MA
      Cancer biology & therapy  15  840-50  2014

      Show Abstract
      24755837 24755837
      Wild-type p53-induced phosphatase 1 (Wip1) forestalls cellular premature senescence at physiological oxygen levels by regulating DNA damage response signaling during DNA replication.
      Sakai, H; Fujigaki, H; Mazur, SJ; Appella, E
      Cell cycle (Georgetown, Tex.)  13  1015-29  2014

      Show Abstract
      Immunocytochemistry24552809 24552809
      Regulation of intra-S phase checkpoint by ionizing radiation (IR)-dependent and IR-independent phosphorylation of SMC3.
      Luo, H; Li, Y; Mu, JJ; Zhang, J; Tonaka, T; Hamamori, Y; Jung, SY; Wang, Y; Qin, J
      The Journal of biological chemistry  283  19176-83  2008

      Show Abstract
      18442975 18442975
      Differential roles for checkpoint kinases in DNA damage-dependent degradation of the Cdc25A protein phosphatase.
      Jin, J; Ang, XL; Ye, X; Livingstone, M; Harper, JW
      The Journal of biological chemistry  283  19322-8  2008

      Show Abstract
      18480045 18480045
      Delayed mammary gland involution in MMTV-AKT1 transgenic mice.
      Ackler, S; Ahmad, S; Tobias, C; Johnson, MD; Glazer, RI
      Oncogene  21  198-206  2002

      Show Abstract
      11803463 11803463
      Specific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation.
      James, SR; Downes, CP; Gigg, R; Grove, SJ; Holmes, AB; Alessi, DR
      The Biochemical journal  315 ( Pt 3)  709-13  1996

      Show Abstract
      8645147 8645147
      Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.
      Burgering, BM; Coffer, PJ
      Nature  376  599-602  1995

      Show Abstract
      7637810 7637810


      NPI Flyer- Epigenetics and Nuclear Function Feature

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      Life Science Research > Antibodies and Assays > Primary Antibodies