NE1037 Anti-APOC1 Mouse mAb (2E2-1A3)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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NE1037-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~10 kDa APOC1 protein in human liver tissue.
      Catalogue NumberNE1037
      Brand Family Calbiochem®
      SynonymsAnti-Apolipoprotein
      Application Data
      Detection of human APOC1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-APOC1 Mouse mAb (2E2-1A3) (Cat. No. NE1037) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesAndersson, B., et al. 2009. Proteome Sci. 7, 40.
      Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-84 of human APOC1, expressed as a GST fusion protein
      Clone2E2-1A3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-APOC1 Mouse mAb (2E2-1A3) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Andersson, B., et al. 2009. Proteome Sci. 7, 40.
      Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Apolipoprotein
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human APOC1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-APOC1 Mouse mAb (2E2-1A3) (Cat. No. NE1037) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~10 kDa APOC1 protein.
      BackgroundAPOC1 is a member of the apolipoprotein C1 family. This protein is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within an apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-84 of human APOC1, expressed as a GST fusion protein
      Clone2E2-1A3
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman liver tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesAndersson, B., et al. 2009. Proteome Sci. 7, 40.
      Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.