Product Information
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
Clone1G7
HostMouse
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-AFP Mouse mAb (1G7) SDS

Title

Safety Data Sheet (SDS) 

Anti-AFP Mouse mAb (1G7) Certificates of Analysis

TitleLot Number
ST1673
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-α-Fetoprotein
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
BackgroundThis gene encodes α-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. α-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of α-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the α-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. α-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of α-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
Clone1G7
IsotypeIgG
Specieshuman
Positive controlHepG2 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies