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MABE1093 Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone EDL HMC 1A

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MABE1093
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1093
      DescriptionAnti-5-Hydroxymethylcytosine (5hmC) Antibody, clone EDL HMC 1A
      Alternate Names
      • 5hmC
      • 5-hmC
      Background Information5-Hydroxymethylcytosine (also known as 5-hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). 5hmC is a DNA pyrimidine nitrogen base and is a predominantly stable DNA modification. It is produced by the action of three Ten-eleven translocation 1 (TET1) enzyme on 5mC. Each mammalian cell contains 5hmC, but its levels vary significantly depending on the cell type. Higher levels of 5hmC are found in neuronal cells and its level is shown to increase with age in hippocampus and cerebellum. It is also prevalent in embryonic stems cells and its reduced levels are shown to result in impaired self-renewal of stem cells. In the brain and in embryonic stem cells, 5hmC in enriched in promoters, gene bodies, and intergenic areas near genes and positively correlates with gene expression at these loci. 5hmC appears to play a role to promote gene expression during active demethylation. Significantly lower of 5hmC have been reported in cancer cell lines, melanoma, adenomas, and carcinomas compared to the normal surrounding tissue.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-5-Hydroxymethylcytosine (5hmC), clone EDL HMC 1A, Cat. No. MABE1093, is a highly specific mouse monoclonal antibody that targets DNA with 5-hydroxymethylcytosine (5hmC) modification and has been tested in Dot Blot and Immunoprecipitation.
      Key Applications
      • Immunoprecipitation
      • Dot Blot
      Application NotesDot Blot Analysis: 0.5 µg/mL from a representative lot detected 0.39-100 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).

      Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated 5 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).
      Biological Information
      ImmunogenBSA-conjugated 5-hydroxymethylcytidine.
      CloneEDL HMC 1A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Species Reactivity NoteAll. Target modification is not species-specific.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot analysis of DNA cytosine modifications.

      Dot Blot Analysis: 0.2 µg/mL of this antibody detected 50 ng of DNA with 5-hydroxymethylcytosine (5hmC), but not unmodified DNA or DNA with only 5-methylcytosine (5mC) modification.


      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1093 04054839134326

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