ST1053 Anti-20S Proteasome Core Subunits Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, Yeast Rb Polyclonal Antibody

Pricing & Availability

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ST1053-100UL
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      Description
      OverviewRecognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
      Catalogue NumberST1053
      Brand Family Calbiochem®
      Application Data
      Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationSerum diluted in PBS.
      Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1:1000-1:10,000, see application references)
      Immunoprecipitation (see comments)
      Paraffin Sections (1:100)
      Application CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman erythrocyte proteasomes
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Yeast
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-20S Proteasome Core Subunits Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-20S Proteasome Core Subunits Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1053
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-July-2007
      ApplicationImmunoblotting (1:1000-1:10,000, see application references)
      Immunoprecipitation (see comments)
      Paraffin Sections (1:100)
      Application Data
      Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).
      BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The β-subunits are believed to be responsible for the proteolytic activity of the 20S proteasome.
      HostRabbit
      Immunogen speciesHuman
      Immunogenhuman erythrocyte proteasomes
      IsotypeIgG
      Specieshuman, mouse, yeast
      Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
      FormLiquid
      FormulationSerum diluted in PBS.
      Preservative≤0.1% sodium azide
      CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      Application referencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.